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Volume 27, Number 5—May 2021
Research

Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology

Thai Leong Yap1, Shin Yee Hong1, Jun Hui Soh, Lekha Ravichandraprabhu, Vanessa W.X. Lim, Hsi-Min Chan, Tommy Z.X. Ong, Ying Ping Chua, Shi En Koh, Huajing Wang, Yee Sin Leo, Jackie Y. Ying, and William SunComments to Author 
Author affiliations: Experimental Drug Development Centre, Singapore (T.L. Yap, S.Y. Hong); Institute of Bioengineering and Nanotechnology, Singapore (T.L. Yap, S.Y. Hong, L. Ravichandraprabhu, T.Z.X. Ong, Y.P. Chua, S.E. Koh, H. Wang, W. Sun); NanoBio Lab, Singapore (J.H. Soh, H.-M. Chan, J.Y. Ying); National Centre for Infectious Diseases, Singapore (V.W.X. Lim, Y.S. Leo); Tan Tock Seng Hospital, Singapore (Y.S. Leo); Yong Loo Lin School of Medicine, National University of Singapore (Y.S. Leo, W. Sun).

Main Article

Table 3

Sensitivity and specificity comparison between GenBody and in-house IA assays for study of Zika diagnosis, Singapore*

Late phase, 7–16 dpo Sensitivity, % (95% CI)
 Specificity, % (95% CI)
GenBody Lateral flow
 GenBody Lateral flow
F1 F2 F1 F2
IgM 28.6 (15.7–44.6) 76.2 (60.5–87.9) 73.8 (58.0–86.1) 97.4 (86.5–99.9) 100.0 (91.0–100.0) 94.9 (82.7–99.4)
IgG 71.4 (55.4–84.3) 85.7 (71.4–94.6) 76.2 (60.5–87.9) 61.5 (44.6–76.6) 79.5 (63.5–90.7) 89.7 (75.8–97.1)
IgM/IgG 78.6 (63.2–89.7) 85.7 (71.4–94.6) 85.7 (71.5–94.6) 56.0 (42.1–74.4) 79.5 (63.5–90.7) 84.6 (69.5–94.1)

*All IA assays were evaluated with TTSH plasma for IgM and IgG test (ZIKV, n = 42; DENV, n = 39, 7–16 dpo, subset of blinded test samples). GenBody strips were tested in a nonblinded approach, and compared with F1 and F2 results that were obtained from the blinded test of the validation set. DENV, dengue virus; dpo, days post onset of symptoms; F1, capture format; F2, detector format; IA, immunochromatographic assay; TTSH, Tan Tock Seng Hospital; ZIKV, Zika virus.

Main Article

1These authors contributed equally to this article.

Page created: April 05, 2021
Page updated: April 20, 2021
Page reviewed: April 20, 2021
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