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Volume 29, Number 8—August 2023

Research

Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment

Egie E. Enabulele, Winka Le Clec’h, Emma K. Roberts, Cody W. Thompson, Molly M. McDonough, Adam W. Ferguson, Robert D. Bradley, Timothy J. C. Anderson, and Roy N. PlattComments to Author 
Author affiliations: Texas Biomedical Research Institute, San Antonio, Texas, USA (E.E. Enabulele, W. Le Clec’h, T.J.C. Anderson, R.N. Platt II); Texas Tech University, Lubbock, Texas, USA (E.K. Roberts, R.D. Bradley); University of Michigan, Ann Arbor, Michigan, USA (C.W. Thompson); Chicago State University, Chicago, Illinois, USA (M.M. McDonough); Field Museum of Natural History, Chicago (A.W. Ferguson)

Main Article

Figure 5

Phylogenetic analysis of pathogens used in control samples for study of prospecting for zoonotic pathogens by using targeted DNA enrichment. A) Schistosoma; B) Plasmodium; C) Mycobacterium. Reads from each control pathogen (M. tuberculosis, P. falciparum, P. vivax, and S. mansoni) were extracted, assembled, aligned, and trimmed for maximum-likelihood phylogenetic analyses. The phylogenies were used to identify the species or strain of pathogen used in the controls. Blue indicates control samples. Bootstrap support values are indicated by colored diamonds at each available node. Branches with <50% bootstrap support were collapsed. Nodal support is indicated by color coded diamonds. Scale bars indicate nucleotide substitutions per site. Assembly accession numbers (e.g., GCA902374465) and tree files are available from https://doi.org/10.5281/zenodo.8014941.

Figure 5. Phylogenetic analysis of pathogens used in control samples for study of prospecting for zoonotic pathogens by using targeted DNA enrichment. A) Schistosoma; B) Plasmodium; C) Mycobacterium. Reads from each control pathogen (M. tuberculosis, P. falciparum, P. vivax, and S. mansoni) were extracted, assembled, aligned, and trimmed for maximum-likelihood phylogenetic analyses. The phylogenies were used to identify the species or strain of pathogen used in the controls. Blue indicates control samples. Bootstrap support values are indicated by colored diamonds at each available node. Branches with <50% bootstrap support were collapsed. Nodal support is indicated by color coded diamonds. Scale bars indicate nucleotide substitutions per site. Assembly accession numbers (e.g., GCA902374465) and tree files are available from https://doi.org/10.5281/zenodo.8014941.

Main Article

Page created: June 07, 2023
Page updated: July 20, 2023
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