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Volume 29, Number 8—August 2023
Research

Prospecting for Zoonotic Pathogens by Using Targeted DNA Enrichment

Egie E. Enabulele, Winka Le Clec’h, Emma K. Roberts, Cody W. Thompson, Molly M. McDonough, Adam W. Ferguson, Robert D. Bradley, Timothy J. C. Anderson, and Roy N. PlattComments to Author 
Author affiliations: Texas Biomedical Research Institute, San Antonio, Texas, USA (E.E. Enabulele, W. Le Clec’h, T.J.C. Anderson, R.N. Platt II); Texas Tech University, Lubbock, Texas, USA (E.K. Roberts, R.D. Bradley); University of Michigan, Ann Arbor, Michigan, USA (C.W. Thompson); Chicago State University, Chicago, Illinois, USA (M.M. McDonough); Field Museum of Natural History, Chicago (A.W. Ferguson)

Main Article

Figure 2

Targeted DNA enrichment workflow for study of prospecting for zoonotic pathogens by using targeted DNA enrichment. A) Genomic DNA extracted using the DNeasy Kit (QIAGEN, https://www.qiagen.com). B) Next-generation sequencing libraries prepared using KAPA Hyperplus Kit (https://www.biocompare.com) and barcoding each library with IDT xGen Stubby Adaptor-UDI Primers (https://www.idtdna.com). C) RNA probes hybridization using the high sensitivity protocol of myBaits version 5. (https://arborbiosci.com). D) Probes bound to streptavidin-coated magnetic beads and sequestered with a magnet (E) 15 cycles PCR amplification of enriched libraries. F) Libraries sequenced on an Illumina Hi-Seq 2500 platform (https://www.illumina.com).

Figure 2. Targeted DNA enrichment workflow for study of prospecting for zoonotic pathogens by using targeted DNA enrichment. A) Genomic DNA extracted using the DNeasy Kit (QIAGEN, https://www.qiagen.com). B) Next-generation sequencing libraries prepared using KAPA Hyperplus Kit (https://www.biocompare.com) and barcoding each library with IDT xGen Stubby Adaptor-UDI Primers (https://www.idtdna.com). C) RNA probes hybridization using the high sensitivity protocol of myBaits version 5. (https://arborbiosci.com). D) Probes bound to streptavidin-coated magnetic beads and sequestered with a magnet (E) 15 cycles PCR amplification of enriched libraries. F) Libraries sequenced on an Illumina Hi-Seq 2500 platform (https://www.illumina.com).

Main Article

Page created: June 07, 2023
Page updated: July 20, 2023
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