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Volume 30, Number 6—June 2024
Research

Lack of Transmission of Chronic Wasting Disease Prions to Human Cerebral Organoids

Bradley R. Groveman1, Katie Williams1, Brent Race, Simote Foliaki, Tina Thomas, Andrew G. Hughson, Ryan O. Walters, Wenquan Zou, and Cathryn L. HaighComments to Author 
Author affiliations: Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA (B.R. Groveman, K. Williams, B. Race, S. Foliaki, T. Tomas, A.G. Hughson, R.O. Walters, C.L. Haigh); Jiangxi Academy of Clinical Medical Sciences, The First Affiliated Hospital of Nanchang University, Nanchang, China (W. Zou)

Main Article

Figure 1

PrestoBlue viability and LDH release assays for the 129MM (A) and 129MV (B) representative organoids measured before harvest at 180 days post innoculation in study of lack of transmission of CWD prions to human cerebral organoids. Results indicate CWD exposure does not reduce organoid viability. Individual dots represent a single organoid, bars indicate the mean response, and error bars show SDs. No condition was statistically changed from controls as determined by 1-way analysis of variance with Welch’s correction. au, arbitrary units; CJD, Creutzfeldt-Jakob disease; CWD, chronic wasting disease; dCWD1, whitetail deer CWD; dCWD2, mule deer CWD; dNBH, deer normal brain homogenate; eCWD, elk CWD; eNBH, elk normal brain homogenate; hNBH, human normal brain homogenate; LDH, lactate dehydrogenase.

Figure 1. PrestoBlue viability and LDH release assays for the 129MM (A) and 129MV (B) representative organoids measured before harvest at 180 days post innoculation in study of lack of transmission of CWD prions to human cerebral organoids. Results indicate CWD exposure does not reduce organoid viability. Individual dots represent a single organoid, bars indicate the mean response, and error bars show SDs. No condition was statistically changed from controls as determined by 1-way analysis of variance with Welch’s correction. au, arbitrary units; CJD, Creutzfeldt-Jakob disease; CWD, chronic wasting disease; dCWD1, whitetail deer CWD; dCWD2, mule deer CWD; dNBH, deer normal brain homogenate; eCWD, elk CWD; eNBH, elk normal brain homogenate; hNBH, human normal brain homogenate; LDH, lactate dehydrogenase.

Main Article

1These authors contributed equally to this article.

Page created: May 02, 2024
Page updated: May 22, 2024
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