Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 30, Number 6—June 2024
Research

Lack of Transmission of Chronic Wasting Disease Prions to Human Cerebral Organoids

Bradley R. Groveman1, Katie Williams1, Brent Race, Simote Foliaki, Tina Thomas, Andrew G. Hughson, Ryan O. Walters, Wenquan Zou, and Cathryn L. HaighComments to Author 
Author affiliations: Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA (B.R. Groveman, K. Williams, B. Race, S. Foliaki, T. Tomas, A.G. Hughson, R.O. Walters, C.L. Haigh); Jiangxi Academy of Clinical Medical Sciences, The First Affiliated Hospital of Nanchang University, Nanchang, China (W. Zou)

Main Article

Figure 4

Western blot analysis of total PrP and PrPres levels in representative organoids for sporadic CJD and CWD-exposed human cerebral organoids in study of lack of transmission of chronic wasting disease prions to 129MM (A) and 129MV (B) human cerebral organoids. Matched KO organoids inoculated with dCWD2 or eCWD are shown for comparison. Densitometric analysis (shown in bottom panels) shows total PrP levels relative to total protein with each point representing an individual organoid; means and SDs are indicated. p values were calculated using Welch’s t-test. Uncropped Western analyses are shown in Appendix Figure 3. CJD, Creutzfeldt-Jakob disease; CWD, chronic wasting disease; dCWD1, whitetail deer CWD; dCWD2, mule deer CWD; dNBH, deer normal brain homogenate; eCWD, elk CWD; eNBH, elk normal brain homogenate; hNBH, human normal brain homogenate; KO, knockout; PrP, prion protein; PrPres, protease-resistant prion protein.

Figure 4. Western blot analysis of total PrP and PrPres levels in representative organoids for sporadic CJD and CWD-exposed human cerebral organoids in study of lack of transmission of chronic wasting disease prions to 129MM (A) and 129MV (B) human cerebral organoids. Matched KO organoids inoculated with dCWD2 or eCWD are shown for comparison. Densitometric analysis (shown in bottom panels) shows total PrP levels relative to total protein with each point representing an individual organoid; means and SDs are indicated. p values were calculated using Welch’s t-test. Uncropped Western analyses are shown in Appendix Figure 3. CJD, Creutzfeldt-Jakob disease; CWD, chronic wasting disease; dCWD1, whitetail deer CWD; dCWD2, mule deer CWD; dNBH, deer normal brain homogenate; eCWD, elk CWD; eNBH, elk normal brain homogenate; hNBH, human normal brain homogenate; KO, knockout; PrP, prion protein; PrPres, protease-resistant prion protein.

Main Article

1These authors contributed equally to this article.

Page created: May 02, 2024
Page updated: May 22, 2024
Page reviewed: May 22, 2024
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external