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Volume 30, Number 6—June 2024
Research

Lack of Transmission of Chronic Wasting Disease Prions to Human Cerebral Organoids

Bradley R. Groveman1, Katie Williams1, Brent Race, Simote Foliaki, Tina Thomas, Andrew G. Hughson, Ryan O. Walters, Wenquan Zou, and Cathryn L. HaighComments to Author 
Author affiliations: Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA (B.R. Groveman, K. Williams, B. Race, S. Foliaki, T. Tomas, A.G. Hughson, R.O. Walters, C.L. Haigh); Jiangxi Academy of Clinical Medical Sciences, The First Affiliated Hospital of Nanchang University, Nanchang, China (W. Zou)

Main Article

Figure 2

Demonstration of infection and accumulation of MV2 sporadic CJD prions in human cerebral organoids in study of lack of transmission of chronic wasting disease prions to human cerebral organoids. Both the 129MM and MV organoids were infected with MV2 sporadic CJD prions to ensure uptake and accumulation could be measured in both lines. A) Real-time quaking-induced conversion seeding activity; B) accumulation of protease resistant prion protein were assayed at 56 and 180 dpi. Each marker in panel A represents the organoid from an individual with the means and SDs of all organoids per condition indicated. Panel B indicates Western blots using prion 3F4 antibody following protease digest of lysates from 2 representative MM and 2 representative MV organoids that received the same starting inoculum (MV2 CJD) along with a 129MM 180dpi organoid that received hNBH. CJD, Creutzfeldt-Jakob disease; dpi, days postinoculation; hNBH, human normal brain homogenate.

Figure 2. Demonstration of infection and accumulation of MV2 sporadic CJD prions in human cerebral organoids in study of lack of transmission of chronic wasting disease prions to human cerebral organoids. Both the 129MM and MV organoids were infected with MV2 sporadic CJD prions to ensure uptake and accumulation could be measured in both lines. A) Real-time quaking-induced conversion seeding activity; B) accumulation of protease resistant prion protein were assayed at 56 and 180 dpi. Each marker in panel A represents the organoid from an individual with the means and SDs of all organoids per condition indicated. Panel B indicates Western blots using prion 3F4 antibody following protease digest of lysates from 2 representative MM and 2 representative MV organoids that received the same starting inoculum (MV2 CJD) along with a 129MM 180dpi organoid that received hNBH. CJD, Creutzfeldt-Jakob disease; dpi, days postinoculation; hNBH, human normal brain homogenate.

Main Article

1These authors contributed equally to this article.

Page created: May 02, 2024
Page updated: May 22, 2024
Page reviewed: May 22, 2024
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