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Volume 30, Number 6—June 2024
Research

Lack of Transmission of Chronic Wasting Disease Prions to Human Cerebral Organoids

Bradley R. Groveman1, Katie Williams1, Brent Race, Simote Foliaki, Tina Thomas, Andrew G. Hughson, Ryan O. Walters, Wenquan Zou, and Cathryn L. HaighComments to Author 
Author affiliations: Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA (B.R. Groveman, K. Williams, B. Race, S. Foliaki, T. Tomas, A.G. Hughson, R.O. Walters, C.L. Haigh); Jiangxi Academy of Clinical Medical Sciences, The First Affiliated Hospital of Nanchang University, Nanchang, China (W. Zou)

Main Article

Table

Inoculum details used in the study of attempted CWD prion transmission to human cerebral organoids*

Sample Name log LD50/mg brain† log SD50/mg brain‡
Human normal brain homogenate hNBH Negative Negative
Human sporadic CJD MV2 CJD ND 6.9
Deer normal brain homogenate dNBH Negative Negative
Whitetail deer CWD§ (pool of 7) dCWD1 5.6 (WTD) 6
Mule deer CWD¶ (pool of 6) dCWD2 5.7 (MD) 6.2
Elk normal brain homogenate eNBH Negative Negative
Elk CWD# (pool of 6) eCWD 5.3 6.5

*CJD, Creutzfeldt-Jakob disease; CWD, chronic wasting disease; LD50, 50% lethal dose; negative, negative control; ND, not done; SD50, 50% seeding dose. †Adapted from Race et al. (29). ‡Inocula. §WTD-1 in nomenclature as found in (29). ¶MD-1 in nomenclature as found in (29) #Elk-2 in nomenclature as found in (29).

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1These authors contributed equally to this article.

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