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Volume 30, Number 6—June 2024
Research

Lack of Transmission of Chronic Wasting Disease Prions to Human Cerebral Organoids

Bradley R. Groveman1, Katie Williams1, Brent Race, Simote Foliaki, Tina Thomas, Andrew G. Hughson, Ryan O. Walters, Wenquan Zou, and Cathryn L. HaighComments to Author 
Author affiliations: Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA (B.R. Groveman, K. Williams, B. Race, S. Foliaki, T. Tomas, A.G. Hughson, R.O. Walters, C.L. Haigh); Jiangxi Academy of Clinical Medical Sciences, The First Affiliated Hospital of Nanchang University, Nanchang, China (W. Zou)

Main Article

Figure 5

Prion protein (PrP) deposits in organoids. A) Deposits of PrP in 129MM and KO organoids detected with SAF32 antibody in study of lack of transmission of chronic wasting disease prions to human cerebral organoids. B) Prion deposition in sequential slices of representative 129MV organoids is detected by both SAF32 (i) and F89 (ii) total PrP antibodies in both CWD and CJD inoculated organoids. Cervid PrP-specific antibody F99 (iii) detects the same deposits in the just the CWD inoculated organoids, indicating that the deposits are of cervid origin (i.e., inocula) and not misfolded human PrP. Scale bars indicate 50 µm. CJD, Creutzfeldt-Jakob disease; CWD, chronic wasting disease; dCWD2, mule deer CWD; dNBH, deer normal brain homogenate; eCWD, elk CWD; KO, knockout.

Figure 5. Prion protein (PrP) deposits in organoids. A) Deposits of PrP in 129MM and KO organoids detected with SAF32 antibody in study of lack of transmission of chronic wasting disease prions to human cerebral organoids. B) Prion deposition in sequential slices of representative 129MV organoids is detected by both SAF32 (i) and F89 (ii) total PrP antibodies in both CWD and CJD inoculated organoids. Cervid PrP-specific antibody F99 (iii) detects the same deposits in the just the CWD inoculated organoids, indicating that the deposits are of cervid origin (i.e., inocula) and not misfolded human PrP. Scale bars indicate 50 µm. CJD, Creutzfeldt-Jakob disease; CWD, chronic wasting disease; dCWD2, mule deer CWD; dNBH, deer normal brain homogenate; eCWD, elk CWD; KO, knockout.

Main Article

1These authors contributed equally to this article.

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Page updated: May 22, 2024
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