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Volume 32, Number 5—May 2026

Dispatch

Development and Validation of Real-Time PCR for Detecting Anaplasma bovis–Like Agent in Dermacentor spp. Ticks

Rachel C. SmithComments to Author , Daniel F. Barrantes Murillo, Aryanna Carr, Kathryn T. Duncan, and Lindsay A. Starkey
Author affiliation: Oklahoma State University College of Veterinary Medicine, Stillwater, Oklahoma, USA

Main Article

Figure 1

Results of the standard curve analyses, correlation, and amplification efficiency of real-time PCR for detecting Anaplasma bovis–like agent in Dermacentor spp. ticks, United States. We conducted standard curve analyses to evaluate assay efficiency and sensitivity by using 2 sets of samples: a 10-fold dilution series containing synthetic A. bovis–like DNA only and the same dilution series with ≈54 ng of noninfected D. variabilis tick DNA added per reaction. We used tick samples to assess assay performance in the tick DNA matrix. Graph shows standard curve lines for each sample set, with cycle threshold value graphed as a function of target copy number.

Figure 1. Results of the standard curve analyses, correlation, and amplification efficiency of real-time PCR for detecting Anaplasma bovis–like agent in Dermacentor spp. ticks, United States. We conducted standard curve analyses to evaluate assay efficiency and sensitivity by using 2 sets of samples: a 10-fold dilution series containing synthetic A. bovis–like DNA only and the same dilution series with ≈54 ng of noninfected D. variabilis tick DNA added per reaction. We used tick samples to assess assay performance in the tick DNA matrix. Graph shows standard curve lines for each sample set, with cycle threshold value graphed as a function of target copy number.

Main Article

Page created: April 28, 2026
Page updated: May 07, 2026
Page reviewed: May 07, 2026
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