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Volume 32, Number 5—May 2026
Dispatch
Development and Validation of Real-Time PCR for Detecting Anaplasma bovis–Like Agent in Dermacentor spp. Ticks
Rachel C. Smith
, Daniel F. Barrantes Murillo, Aryanna Carr, Kathryn T. Duncan, and Lindsay A. Starkey
, Daniel F. Barrantes Murillo, Aryanna Carr, Kathryn T. Duncan, and Lindsay A. Starkey
Table 1
Oligo sequences used to develop and validate real-time PCR for detecting Anaplasma bovis–like agent in Dermacentor spp. ticks, United States*
| Type | Oligo name | Sequence, 5′ → 3′ |
|---|---|---|
| Primer |
AboviTMF |
TGC GCA GTG TGT TAA GGA AG |
| Primer |
AboviTMR |
ACG GAG AAA GAT ATC CAC GAT CA |
| Probe | AboviTMPro | FAM-TCG GAA GAG ACG GAG TAA-MGB |
*Oligos devised from heat shock chaperon gene (groEL) sequences from A. bovis–like agent and related Anaplasma spp. bacteria retrieved from GenBank (Appendix Table).
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