Volume 32, Number 5—May 2026
Dispatch
Development and Validation of Real-Time PCR for Detecting Anaplasma bovis–Like Agent in Dermacentor spp. Ticks
Rachel C. Smith
, Daniel F. Barrantes Murillo, Aryanna Carr, Kathryn T. Duncan, and Lindsay A. Starkey
, Daniel F. Barrantes Murillo, Aryanna Carr, Kathryn T. Duncan, and Lindsay A. Starkey
Table 2
Results from survey of ticks tested with real-time PCR for detecting Anaplasma bovis–like agent in Dermacentor spp. ticks, United States*
| Tick species | Tick host |
Tick sex |
No. ticks tested | No. PCR positive (% [95% CI]) | |||
|---|---|---|---|---|---|---|---|
| Dog | Cat | F | M | ||||
| D. variabilis | 592 | 39 | 394 | 237 | 631 | 1 (0.2 [0–0.9]) | |
| D. andersoni | 35 | 2 | 30 | 7 | 37 | 0 (0 [0–9.5]) | |
| D. albipictus |
2 |
2 |
3 |
1 |
4 |
0 (0 [0–60.2]) |
|
| Total | 629 | 43 | 427 | 245 | 672 | 1 (0.1 [0–0.8]) | |
*Ticks were collected at veterinary clinics across the United States during 2021–2023 and shipped to the Oklahoma State University College of Veterinary Medicine Show Us Your Ticks program (https://www.showusyourticks.org), where staff morphologically identified ticks and stored in 70% ethanol at −20°C until dissection and DNA extraction in this study.
Page created: April 28, 2026
Page updated: May 07, 2026
Page reviewed: May 07, 2026
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