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Volume 32, Number 8—August 2026
Research
Detection of Highly Pathogenic Avian Influenza A(H5N1) Clade 2.3.4.4b Genotype D1.2 Virus in Swine after Experimental Inoculation
Table 2
Necropsy Ct values for tissue sample from pigs in study of systemic distribution and protracted detection of highly pathogenic avian influenza A(H5N1) clade 2.3.4.4b genotype D1.2 virus in swine after experimental inoculation*
| Group and pig ID | Sample source |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| BALF | Brain | Heart | Lung, homogenized | Diaphragm | Meat juice | Ileum swab | Spiral colon swab | Pancreas | Spleen | |
| D1.2/OR, necropsy at 5 DPI | ||||||||||
| 641 | 32.4 | 30.1 | 40.0 | 38.6 | 37.4 | 32.0 | 40.0 | 36.0 | 40.0 | 34.7 |
| 642 | 40.0 | 39.8 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 39.1 |
| 643 | 33.1 | 33.6 | 40.0 | 37.7 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 |
| 644 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 |
| 645 | 30.6 | 36.3 | 40.0 | 40.0 | 38.5 | 31.2 | 39.2 | 36.1 | 40.0 | 35.0 |
| 646 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 |
| 647 | 30.4 | 39.8 | 40.0 | 27.5 | 40.0 | 36.4 | 40.0 | 40.0 | 40.0 | 40.0 |
| 648 |
32.7 |
35.8 |
40.0 |
40.0 |
40.0 |
40.0 |
39.8 |
40.0 |
40.0 |
40.0 |
| D1.2/OR, necropsy at 35 DPI | ||||||||||
| 649 | 40.0 | 40.0 | 40.0 | 38.2 | 40.0 | 40.0 | 40.0 | 37.5 | 40.0 | – |
| 650 | 40.0 | 38.4 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | – |
| 651 |
40.0 |
40.0 |
40.0 |
40.0 |
40.0 |
40.0 |
40.0 |
40.0 |
40.0 |
– |
| Control, necropsy at 5 DPI | ||||||||||
| 652 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 |
| 653 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 |
| 654 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 | 40.0 |
*Animals were humanely euthanized at day 5 or 35 DPI. Tissues or swabs taken at necropsy at 5 or 35 DPI. Samples were tested by quantitative reverse transcription PCR. Light gray shading indicates samples with positive results (Ct <38.0); dark gray shading indicates a suspected result (Ct 38.0–40.0). Samples with a Ct value <35 were selected for virus isolation; bold indicates samples from which virus was isolated. Dashes indicate missing samples. Ct, cycle threshold; DPI, days postinoculation.