Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 20, Number 5—May 2014

Bovine Leukemia Virus DNA in Human Breast Tissue

Gertrude Case BuehringComments to Author , Hua Min Shen, Hanne M. Jensen, K. Yeon Choi1, Dejun Sun, and Gerard Nuovo
Author affiliations: University of California, Berkeley, California, USA (G.C. Buehring, H.M. Shen, K.Y. Choi, D. Sun); University of California Davis Medical Center, Sacramento, California, USA (H.M. Jensen); Ohio State University Comprehensive Cancer Center, Columbus, Ohio, USA (G. Nuovo)

Main Article

Table 2

Lack of cross-reactivity of anti-BLV p24 (capsid region) mAb with common chronic viruses in human tissues*

Virus Cells or tissue producing virus (source†) Type of antibody to virus (source†) Reaction of cells or tissue with
Ab to virus mAb to BLV p24
BLV FLK (positive control) (K. Radke) mAb to BLV p24 (ARRRP no. 12145) + +
Bat2Cl6 (positive control) (K. Radke) + +
None Tb1Lu (negative control) (K. Radke)
MMTV GR (G. Firestone) pAb to MMTV (NCI-BCP) +
HBV Infected human liver sections (Dako) pAb to HBV (Dako) +
HTLV-1 MT-2 (C. Hanson) pAb to HTLV-1 (ARRRP) +
HTLV-2 Clone-19 (C. Hanson) pAb to HTLV-2 (ARRRP) +
HIV-1, -2 H9 HIV-1, -2 (C. Hanson) pAb to HIV-1, -2 (ARRRP) +
HPV HPV-infected keratinocyte cultures
(C. Meyers) pAb to PV (Dako) +
EBV B95–8 (CCL/NBRL) pAb to EBV (Dako) +
CMV Infected human lung sections (Dako) pAb to CMV (Chemicon/Millipore) +
HHV 1, 2 Infected human cells (Syva) pAb to HHV 1, 2 (Chemicon/Millipore) +

*BLV, bovine leukemia virus; mAb, monoclonal antibody; Ab, antibody; FLK, fetal lamb kidney; MMTV, mouse mammary tumor virus; pAb, polyclonal antibody; HBV, hepatitis B virus; HTLV, human T cell leukemia virus; HPV, human papillomavirus; PV, papillomavirus; EBV, Epstein-Barr virus; CMV, cytomegalovirus; HHV, human herpes virus.
†Individually named sources listed in Acknowledgments. ARRRP, AIDS Research and Reference Reagent Program, National Institute of Allergy and Infectious Diseases (; NCI-BCP, National Cancer Institute, Breast Cancer Program; Dako, Glostrup, Denmark; CCL/NBRL, former Cell Culture Laboratory of the Naval Bioscience Research Laboratory, Oakland, CA, USA; Chemicon/Millipore, Ballerica, MA; Syva, Palo Alto, CA, USA.

Main Article

1Current affiliation: Texas A&M Health Science Center College of Medicine, College Station, Texas, USA.

Page created: April 16, 2014
Page updated: April 16, 2014
Page reviewed: April 16, 2014
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.