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Volume 21, Number 1—January 2015

Research

Protocol for Metagenomic Virus Detection in Clinical Specimens1

Claudia KohlComments to Author , Annika Brinkmann, Piotr W. Dabrowski, Aleksandar Radonić, Andreas Nitsche, and Andreas Kurth
Author affiliations: Robert Koch Institute, Berlin, Germany

Main Article

Figure 2

Validation of test aliquots of infected mode used for development of tissue-based universal virus detection for viral metagenomics protocol. Every ninth aliquot was extracted, and viral copy numbers were determined by using a quantitative PCR. Standard deviations (error bars), medians (solid horizontal lines), and residual plots indicate homogeneity and mixture of test specimens. Ct, cycle threshold.

Figure 2. Validation of test aliquots of infected mode used for development of tissue-based universal virus detection for viral metagenomics protocol. Every ninth aliquot was extracted, and viral copy numbers were determined by using a quantitative PCR. Standard deviations (error bars), medians (solid horizontal lines), and residual plots indicate homogeneity and mixture of test specimens. Ct, cycle threshold.

Main Article

1Preliminary results of this study were presented at the Biodefense and Emerging Infectious Diseases Meeting, January 29, 2014, Washington DC, USA.

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Page updated: January 05, 2015
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