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Volume 21, Number 1—January 2015

Protocol for Metagenomic Virus Detection in Clinical Specimens1

Claudia KohlComments to Author , Annika Brinkmann, Piotr W. Dabrowski, Aleksandar Radonić, Andreas Nitsche, and Andreas Kurth
Author affiliations: Robert Koch Institute, Berlin, Germany

Main Article

Table 3

Properties of 4 viruses used to develop a protocol for metagenomic virus detection in infectious disease settings*

Reovirinae, reovirus
Orthomyxovirinae, influenza virus A
Poxvirinae, vaccinia virus
Paramyxovirinae, Sendai virus
Size, nm, shape 75–85, icosahedral 80–120, spherical, pleomorphic 270 × 350, brick-shaped complex 150–350, spherical, pleomorphic
Buoyant density, g/mL 1.36 1.2 1.23–1.27 1.31
Size genome, kbp ≈23.5 ≈13.5 186–192 ≈15.5
RNA/DNA dsRNA (–) ssRNA dsDNA (–) ssRNA
Genome organization Linear, 10 segments Linear, 8 segments Linear, continuous Linear, continuous
Envelope No Yes Yes Yes
Replication Cytoplasm Nucleus Cytoplasm Cytoplasm
Virion assembly Cytoplasmic inclusion bodies (viral factories) Cytoplasm Cytoplasmic factory areas Cytoplasm
Release After virus-induced cell death Budding from cell membrane Exocytosis, cell lysis Budding from cell membrane
Sensitivity Unknown Cesium chloride, heat, formaldehyde, SDS, ultraviolet light, oxidation compound Unknown Cesium chloride, heat, formaldehyde, SDS, oxidation compound

*Virus data were obtained from King et al. (9) and Tidona and Darai (10). –, negative. SDS, sodium dodecyl sulfate.

Main Article

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1Preliminary results of this study were presented at the Biodefense and Emerging Infectious Diseases Meeting, January 29, 2014, Washington DC, USA.

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