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Volume 21, Number 1—January 2015
Research

Protocol for Metagenomic Virus Detection in Clinical Specimens1

Claudia KohlComments to Author , Annika Brinkmann, Piotr W. Dabrowski, Aleksandar Radonić, Andreas Nitsche, and Andreas Kurth
Author affiliations: Robert Koch Institute, Berlin, Germany

Main Article

Figure 8

Results of comparative next-generation sequencing used for development of tissue tissue-based universal virus detection for viral metagenomics (TUViD-VM) protocol. A) Sample preparation flowchart to generate 4 next-generation sequencing approaches. B) Results obtained for model tissue (chicken) infected with 4 viruses: vaccinia virus (poxvirus) Sendai virus (paramyxovirus), influenza virus (A/PR8/1934), or reovirus (T3/Bat/G/342/08). The x-axis is log-scaled, and normalized read numbers are indi

Figure 8. Results of comparative next-generation sequencing used for development of tissue tissue-based universal virus detection for viral metagenomics (TUViD-VM) protocol. A) Sample preparation flowchart to generate 4 next-generation sequencing approaches. B) Results obtained for model tissue (chicken) infected with 4 viruses: vaccinia virus (poxvirus) Sendai virus (paramyxovirus), influenza virus (A/PR8/1934), or reovirus (T3/Bat/G/342/08). The x-axis is log-scaled, and normalized read numbers are indicated. C) Results of marmoset sample proof of principle, Sendai virus–infected lung tissue. The baseline is log-scaled, and normalized read numbers are indicated.

Main Article

1Preliminary results of this study were presented at the Biodefense and Emerging Infectious Diseases Meeting, January 29, 2014, Washington DC, USA.

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Page updated: January 05, 2015
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