Volume 22, Number 5—May 2016
Research
Plasmodium falciparum K76T pfcrt Gene Mutations and Parasite Population Structure, Haiti, 2006–2009
Table 1
Evaluation of Plasmodium falciparum samples for chloroquine resistance by restriction-endonuclease digestion and sequencing, Haiti, 2006–2009*
Source | No. samples | No. pfcrt K76T Apo1 sensitive | No. pfcrt K76T Apo1 combined | No. pfcrt K76T sequenced CVMNK | No. pfcrt K76T sequenced CVMNK/CVIET |
---|---|---|---|---|---|
Jeremie | 308 | 73 | 1† | 234 | 1† |
Jacmel | 36 | 14 | 0 | 22 | 0 |
Les Cayes | 316 | 40 | 1‡ | 275 | 1‡ |
Miragoane | 15 | 7 | 0 | 8 | 0 |
Cap-Haitien | 4 | 2 | 0 | 2 | 0 |
Deschappelles | 199 | 20 | 0 | 179 | 0 |
Fort-Liberte | 13 | ND | NA | 13 | 0 |
Port-de-Paix | 3 | ND | NA | 3 | 0 |
Port-au-Prince |
7 |
ND |
NA |
7 |
0 |
Total | 901 | 156 | 2 | 743 | 2 |
*Sequenced includes sequencing of the nested PCR product for pfcrt haplotypes where wild-type haplotype = CVMNK, and mutant haplotype = CVIET;
ND, not determined; NA, not applicable; pfcrt, P. falciparum chloroquine resistance transporter.
†Same sample from Jeremie.
‡Same sample from Les Cayes; pfcrt K76T ApoI includes endonuclease digestion and gel electrophoresis. Sensitive: K76; resistant: 76T; pfcrt K76T.