Volume 22, Number 8—August 2016
Research
Virulence and Evolution of West Nile Virus, Australia, 1960–2012
Table 2
Binding patterns of monoclonal antibodies to WNV strains in ELISA*
Strain | Year of isolation | Monoclonal antibodies, by specificity |
|||||||
---|---|---|---|---|---|---|---|---|---|
Panflavivirus, 4G2, anti-env | Pan-WNV, 2B2, anti-env | WNVKUN-specific |
Glycosylated E, 17D7 | Unglycosylated E, 3.101C | MVEV-specific, 10C6 | ||||
10A1, anti-env | 5D4, anti-NS5 | 5H1, anti-NS5 | |||||||
WNVKUN† | |||||||||
KUN1960 | 1960 | + | + | + | + | + | – | + | – |
Boort | 1984 | + | + | + | + | + | + | – | – |
K2499 | 1984 | + | + | + | + | + | + | – | – |
SH183 | 1991 | + | + | + | + | + | + | – | – |
K6453 | 1991 | + | + | + | + | + | + | – | – |
Hu6774 | 1991 | + | + | + | + | + | + | – | – |
Gu0631 | 2000 | + | + | + | + | + | + | – | – |
Gu1009 | 2000 | + | + | + | + | + | + | – | – |
K68967 | 2009 | + | + | + | + | – | + | – | – |
P9974 | 2009 | + | + | + | + | – | + | – | – |
NSW2011 | 2011 | + | + | + | + | – | + | – | – |
K74015 | 2011 | + | + | + | + | – | + | – | – |
NSW2012 |
2012 |
+ |
+ |
+ |
+ |
– |
+ |
– |
– |
Reference | |||||||||
WNVNY99 | 1999 | + | + | – | + | – | + | – | – |
MVEV1–51 | 1951 | + | – | – | – | – | – | – | + |
*Binding of a monoclonal antibody was designated as positive if the optical density was at least double the optical density of the negative control (mock-infected C6/36 cells). E, envelope protein; MVEV, Murray Valley encephalitis virus; NS5, nonstructural protein 5; WNV, West Nile virus; WNVKUN, Kunjin strain of WNV; +, positive; –, negative.
†WNVKUN strains collected during 1960–2012, Australia.
1Current affiliation: QIMR Berghofer Medical Research Institute, Herston, Queensland, Australia.
2These authors contributed equally to the major technical aspects of the work.
3Current affiliation: Willamette University, Salem, Oregon, USA.
4These authors served as joint senior authors.