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Volume 23, Number 2—February 2017

Research

Swine Influenza Virus (H1N2) Characterization and Transmission in Ferrets, Chile

Nicolás Bravo-Vasquez1, Erik A. Karlsson1, Pedro Jimenez-Bluhm, Victoria Meliopoulos, Bryan Kaplan, Shauna Marvin, Valerie Cortez, Pamela Freiden, Melinda A. Beck, and Stacey Schultz-Cherry2Comments to Author 
Author affiliations: University of Chile, Santiago, Chile (N. Bravo-Vasquez, C. Hamilton-West); St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (E.A. Karlsson, P. Jimenez-Bluhm, V. Meliopoulos, B. Kaplan, S. Marvin, V. Cortez, P. Freiden, S. Schultz-Cherry); University of North Carolina, Chapel Hill, North Carolina, USA (M.A. Beck)

Main Article

Figure 4

Replication of influenza viruses in vitro. MDCK cells (A), primary normal human bronchial epithelial cells (B), or primary swine nasal epithelial cells (C) were grown in an air-liquid interface and infected with the indicated viruses at a multiplicity of infection of 0.01. Cell culture supernatants were collected at 6 hours postinfection (hpi), 24 hpi, 48 hpi, and 72 hpi, for MDCK cells and 6 hpi, 24 hpi, 48 hpi, 72 hpi, and 96 hpi for primary normal human bronchial epithelial cells and primary

Figure 4. Replication of influenza viruses in vitro. MDCK cells (A), primary normal human bronchial epithelial cells (B), or primary swine nasal epithelial cells (C) were grown in an air-liquid interface and infected with the indicated viruses at a multiplicity of infection of 0.01. Cell culture supernatants were collected at 6 hours postinfection (hpi), 24 hpi, 48 hpi, and 72 hpi, for MDCK cells and 6 hpi, 24 hpi, 48 hpi, 72 hpi, and 96 hpi for primary normal human bronchial epithelial cells and primary swine nasal epithelial cells, and viral titers were determined by 50% tissue culture infectious dose (TCID50) analysis. Data are presented as mean titer of 3 replicates ± SEM. *p<0.05 versus sw/Chile virus.

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1These first authors contributed equally to this article.

2These senior authors contributed equally to this article.

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