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Volume 23, Number 2—February 2017

Research

Swine Influenza Virus (H1N2) Characterization and Transmission in Ferrets, Chile

Nicolás Bravo-Vasquez1, Erik A. Karlsson1, Pedro Jimenez-Bluhm, Victoria Meliopoulos, Bryan Kaplan, Shauna Marvin, Valerie Cortez, Pamela Freiden, Melinda A. Beck, and Stacey Schultz-Cherry2Comments to Author 
Author affiliations: University of Chile, Santiago, Chile (N. Bravo-Vasquez, C. Hamilton-West); St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (E.A. Karlsson, P. Jimenez-Bluhm, V. Meliopoulos, B. Kaplan, S. Marvin, V. Cortez, P. Freiden, S. Schultz-Cherry); University of North Carolina, Chapel Hill, North Carolina, USA (M.A. Beck)

Main Article

Figure 6

Evaluation of H1 virus transmission in ferrets. Donor ferrets (black lines; n = 2 ferrets/group) were inoculated with 106 50% tissue culture infectious dose (TCID50) units of H1 influenza virus sw/Chile (A), Mem/87 (B), or sw/IA (C) viruses. Naive ferrets (n = 2) were either placed in the same cage with the infected group (direct contact, blue lines) or housed in separate cages (respiratory transmission, red lines) and nasal washes were collected on the indicated day postinfection for virus quan

Figure 6. Evaluation of H1 virus transmission in ferrets. Donor ferrets (black lines; n = 2 ferrets/group) were inoculated with 106 50% tissue culture infectious dose (TCID50) units of H1 influenza virus sw/Chile (A), Mem/87 (B), or sw/IA (C) viruses. Naive ferrets (n = 2) were either placed in the same cage with the infected group (direct contact, blue lines) or housed in separate cages (respiratory transmission, red lines) and nasal washes were collected on the indicated day postinfection for virus quantification by TCID50 analysis. Lines represent individual animals. Dashed line represents limit of detection of the assay.

Main Article

1These first authors contributed equally to this article.

2These senior authors contributed equally to this article.

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