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Volume 25, Number 1—January 2019

Research

Association of Increased Receptor-Binding Avidity of Influenza A(H9N2) Viruses with Escape from Antibody-Based Immunity and Enhanced Zoonotic Potential

Joshua E. Sealy, Tahir Yaqub, Thomas P. Peacock1, Pengxiang Chang, Burcu Ermetal, Anabel Clements, Jean-Remy Sadeyen, Arslan Mehboob2, Holly Shelton, Juliet E. Bryant, Rod S. Daniels, John W. McCauley, Munir IqbalComments to Author , and Jean-Remy Royal Veterinary CollegeLondonUKSadeyen
Author affiliations: The Pirbright Institute, Pirbright, UK (J.E. Sealy, T.P. Peacock, P. Chang, A. Clements, J.-R. Sadeyen, H. Shelton, M. Iqbal); University of Veterinary and Animal Sciences, Lahore, Pakistan (T. Yaqub, A. Mehboob); The Francis Crick Institute, London (B. Ermetal, R.S. Daniels, J.W. McCauley); Fondation Mérieux, Lyon, France (J.E. Bryant)

Main Article

Table 1

Change in HI titers of influenza A(H9N2) viruses from Pakistan during 2014–2016 viruses compared to UDL-01/08 virus*

HA backbone and substitution Fold change in HI titer (titer)†
SKP-827/16
I116L – (2,048)
P118S – (2,048)
S134L – (2,048)
N135D – (2,048)
N148S – (2,048)
A156V 1‡ (4,096)
R162Q + D262N – (2,048)
G163E – (2,048)
K164N 1‡ (4,096)
180T 4 (128)
A180T + K164N 4 (128)
A180V 4 (128)
N198D (2,048)
D262N (2,048)
S265I
1‡ (4,096)
UDL-01/08
A180T 4 (128)
A180V 4 (128)

*Postinfection polyclonal antiserum raised in a single chicken inoculated with UDL-01/08 was used in all HI assays. Amino acid substitutions were introduced into a contemporary virus HA backbone (SKP-827/16 with A180) or the UDL-01/08 HA backbone and rescued by reverse genetics. HI titers between contemporary viruses and UDL-01/08 virus were compared. HA, hemagglutinin; HI, hemagglutination inhibition; –, no change.
†The homologous HI titer for UDL-01/08 antiserum was 2,048.
‡Indicates fold decrease compared with UDL-01/08.

Main Article

1Current affiliation: Imperial College London, London, UK.

2Current affiliation: Chinese Academy of Agricultural Sciences, Beijing, China.

Page created: December 18, 2018
Page updated: December 18, 2018
Page reviewed: December 18, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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