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Volume 32, Number 1—January 2026

Research

Enhanced Isolation and Detection of COVID-19 in Hospitalized Patients Undergoing Antiviral Therapy

Ranawaka A.P.M. PereraComments to Author , Andrew Marques, Jevon Graham-Wooten, Li Hui Tan, Noam Cohen, Kyle Rodino, Ronald G. Collman, Frederic D. Bushman, and Susan R. Weiss
Author affiliation: Department of Veterans Affairs, Philadelphia, Pennsylvania, USA (R.A.P.M. Perera); University of Pennsylvania, Philadelphia (R.A.P.M. Perera, A. Marques, J. Graham-Wooten, L.H. Tan, N. Cohen, K. Rodino, R.G. Collman, F.D. Bushman, S.R. Weiss)

Main Article

Figure 5

Efficiency and possible routes of infectious viral particle internalization of SARS-CoV-2 in Vero E6, Vero E6 T2, and Vero E6 A2T2 cell lines from study on the enhanced isolation and detection of COVID-19 in hospitalized patients undergoing antiviral therapy. Samples were separated by using sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane for detection of ACE2, TMPRSS2, GAPDH (A) or SARS-CoV-2 spike and nucleocapsid antibodies (B). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ACE2, angiotensin-converting enzyme 2; del, deletion; TMPRSS2, transmembrane protease serine 2; Vero E6 T2, Vero E6 cells expressing TMPRSS2; Vero E6 A2T2, Vero E6 cells expressing both TMPRSS2 and ACE2.

Figure 5. Efficiency and possible routes of infectious viral particle internalization of SARS-CoV-2 in Vero E6, Vero E6 T2, and Vero E6 A2T2 cell lines from study on the enhanced isolation and detection of COVID-19 in hospitalized patients undergoing antiviral therapy. Samples were separated by using sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane for detection of ACE2, TMPRSS2, GAPDH (A) or SARS-CoV-2 spike and nucleocapsid antibodies (B). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ACE2, angiotensin-converting enzyme 2; del, deletion; TMPRSS2, transmembrane protease serine 2; Vero E6 T2, Vero E6 cells expressing TMPRSS2; Vero E6 A2T2, Vero E6 cells expressing both TMPRSS2 and ACE2.

Main Article

Page created: December 30, 2025
Page updated: January 29, 2026
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