Characterization of a Feline Influenza A(H7N2) Virus
, Gongxun Zhong1
, Yuwei Gao1
, Noriko Nakajima1
, Shufang Fan1
, Shiho Chiba, Kathleen M. Deering, Mutsumi Ito, Masaki Imai, Maki Kiso, Sumiho Nakatsu, Tiago J. Lopes, Andrew J. Thompson, Ryan McBride, David L. Suarez, Catherine A. Macken, Shigeo Sugita, Gabriele Neumann, Hideki Hasegawa, James C. Paulson, Kathy L. Toohey-Kurth, and Yoshihiro Kawaoka
Author affiliations: University of Wisconsin–Madison, Madison, Wisconsin, USA (M. Hatta, G. Zhong, Y. Gao, S. Fan, S. Chiba, K.M. Deering, T.J. Lopes, G. Neumann, K.L. Toohey-Kurth, Y. Kawaoka); National Institute of Infectious Diseases, Tokyo, Japan (N. Nakajima, H. Hasegawa); University of Tokyo, Tokyo (M. Ito, M. Imai, M. Kiso, S. Nakatsu, Y. Kawaoka); The Scripps Research Institute, La Jolla, California, USA (A.J. Thompson, R. McBride, J.C. Paulson); US Department of Agriculture, Athens, Georgia, USA (D.L. Suarez); The University of Auckland, Auckland, New Zealand (C. A. Macken); Japan Racing Association, Tochigi, Japan (S. Sugita)
Figure 2. Growth properties of A/feline/NY/16 and A/chicken/NY/99 viruses in mammalian and avian cells at different temperatures. Cells were infected with viruses at a multiplicity of infection of 0.005 and incubated at 33° and 37°C (or at 37°C and 39°C for avian CEF cells). Supernatants were harvested at the indicated time points. Virus titers were determined by use of plaque assays in Madin-Darby canine kidney (MDCK) cells. A549, human lung carcinoma epithelial cells; Clone81, cat kidney fibroblast cells; Fc2Lu, cat lung cells; CEF, chicken embryo fibroblast cells. The species from which the cell lines are derived are shown. The values presented are the averages of 3 independent experiments ±standard deviation. Statistical significance was determined as described in the Technical Appendix. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.
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