Characterization of a Feline Influenza A(H7N2) Virus
, Gongxun Zhong1
, Yuwei Gao1
, Noriko Nakajima1
, Shufang Fan1
, Shiho Chiba, Kathleen M. Deering, Mutsumi Ito, Masaki Imai, Maki Kiso, Sumiho Nakatsu, Tiago J. Lopes, Andrew J. Thompson, Ryan McBride, David L. Suarez, Catherine A. Macken, Shigeo Sugita, Gabriele Neumann, Hideki Hasegawa, James C. Paulson, Kathy L. Toohey-Kurth, and Yoshihiro Kawaoka
Author affiliations: University of Wisconsin–Madison, Madison, Wisconsin, USA (M. Hatta, G. Zhong, Y. Gao, S. Fan, S. Chiba, K.M. Deering, T.J. Lopes, G. Neumann, K.L. Toohey-Kurth, Y. Kawaoka); National Institute of Infectious Diseases, Tokyo, Japan (N. Nakajima, H. Hasegawa); University of Tokyo, Tokyo (M. Ito, M. Imai, M. Kiso, S. Nakatsu, Y. Kawaoka); The Scripps Research Institute, La Jolla, California, USA (A.J. Thompson, R. McBride, J.C. Paulson); US Department of Agriculture, Athens, Georgia, USA (D.L. Suarez); The University of Auckland, Auckland, New Zealand (C. A. Macken); Japan Racing Association, Tochigi, Japan (S. Sugita)
Figure 4. Immunohistochemistry findings in infected cats. Shown are representative sections of nasal turbinates and lungs of cats infected with the indicated viruses on days 3 and 6 postinfection. Three cats per group were infected intranasally with 106 PFU of virus, and tissues were collected on days 3 and 6 postinfection. Type A influenza virus nucleoprotein (NP) was detected by a mouse monoclonal antibody to this protein. For nasal turbinate sections, -: no NP-positive cells, +/−: NP-positive cells detected in 1–2 focal regions, +: NP-positive cells detected in more than two focal regions, 2+: NP-positive cells in large regions. For bronchus and alveolar sections, -: no NP-positive cells, +/−: Up to 5 NP-positive cells, +: Six or more NP-positive cells. NP-positive cells were detected in focal, but not in diffuse bronchial and alveolar sections. For all analyses, the entire sections were evaluated. Scale bars, 50 μm (nasal turbinates), 100 μm (lung).
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