Figure 2. Total ergosterol content of azole-susceptible and azole-resistant Aspergillus fumigatus isolates in Japan. Ergosterol was quantified in the sets of IFM 60814, a susceptible isolate identified on the first date of testing, and IFM 63420, a resistant isolate identified on the third date of testing, both from 1 patient (A); and IFM 63666, IFM 63768, and IFM 63772, all isolated from other patients in the same hospital (B). The results of repeated experiments are expressed as mean ± SD (error bars). The significance of total ergosterol content was determined by the Student t-test (unpaired, unequal variance). A p value <0.05 was considered significant.