Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 29, Number 2—February 2023
Research

Novel Prion Strain as Cause of Chronic Wasting Disease in a Moose, Finland

Julianna L. Sun, Sehun Kim, Jenna Crowell, Bailey K. Webster, Emma K. Raisley, Diana C. Lowe, Jifeng Bian, Sirkka-Liisa Korpenfelt, Sylvie L. Benestad, and Glenn C. TellingComments to Author 
Author affiliations: Colorado State University, Fort Collins, Colorado, USA (J.L. Sun, S. Kim, J. Crowell, B.K. Webster, E.K. Raisley, D.C. Lowe, J. Bian, G.C. Telling); Finnish Food Authority, Helsinki, Finland (S.-L. Korpenfelt); Norwegian Veterinary Institute, Ås, Norway (S.L. Benestad)

Main Article

Figure 1

Transmission properties of Finland moose chronic wasting disease (CWD) prions in GtQ and GtE mice. Survival curves of intracerebrally inoculated GtQ and GtE mice are shown. A–C) Primary transmissions; D–F) secondary transmissions. A, B) Transmission to GtE mice (orange squares) and GtQ mice (magenta squares) of CNS homogenate from M-F1 (A) and LRS tissue homogenate from M-F1 (B). Arrows in GtQ mouse brains 1 and 2 (A) used for serial transmissions (D and E). C) Survival of GtQ mice infected with M-F1 from (A) (magenta squares) compared with Norway moose CWD isolates M-NO1 (green squares), M-NO2 (dotted green squares), and M-NO3 (crossed green squares). D) Serial passage of M-F1 prions from GtQ mouse brain 1 to GtE (orange squares) and GtQ (magenta squares). E) Serial passage of M-F1 prions from GtQ mouse brain 2 to GtE and GtQ mice. F) incubation times in GtQ mice of GtQ-passaged M-F1 (brains 1 and 2) from mice in panels D and E (magenta squares) compared with GtQ-passaged M-NO1 (green squares) and M-NO2 (dotted green squares). CNS, central nervous system; GtE, CWD-susceptible gene-targeted mice in which the prion protein coding sequence was replaced with one encoding glutamate at codon 226; GtQ, CWD-susceptible gene-targeted mice in which the prion protein coding sequence was replaced with one encoding glutamine at codon 226; LRS, lymphoreticular system; M-F1, Finland moose 1, M-NO1: Norway moose 1, M-NO2: Norway moose 2, M-NO3: Norway moose 3; p1, primary transmissions; p2, secondary transmissions.

Figure 1. Transmission properties of Finland moose chronic wasting disease (CWD) prions in GtQ and GtE mice. Survival curves of intracerebrally inoculated GtQ and GtE mice are shown. A–C) Primary transmissions; D–F) secondary transmissions. A, B) Transmission to GtE mice (orange squares) and GtQ mice (magenta squares) of CNS homogenate from M-F1 (A) and LRS tissue homogenate from M-F1 (B). Arrows in GtQ mouse brains 1 and 2 (A) used for serial transmissions (D and E). C) Survival of GtQ mice infected with M-F1 from (A) (magenta squares) compared with Norway moose CWD isolates M-NO1 (green squares), M-NO2 (dotted green squares), and M-NO3 (crossed green squares). D) Serial passage of M-F1 prions from GtQ mouse brain 1 to GtE (orange squares) and GtQ (magenta squares). E) Serial passage of M-F1 prions from GtQ mouse brain 2 to GtE and GtQ mice. F) incubation times in GtQ mice of GtQ-passaged M-F1 (brains 1 and 2) from mice in panels D and E (magenta squares) compared with GtQ-passaged M-NO1 (green squares) and M-NO2 (dotted green squares). CNS, central nervous system; GtE, CWD-susceptible gene-targeted mice in which the prion protein coding sequence was replaced with one encoding glutamate at codon 226; GtQ, CWD-susceptible gene-targeted mice in which the prion protein coding sequence was replaced with one encoding glutamine at codon 226; LRS, lymphoreticular system; M-F1, Finland moose 1, M-NO1: Norway moose 1, M-NO2: Norway moose 2, M-NO3: Norway moose 3; p1, primary transmissions; p2, secondary transmissions.

Main Article

Page created: December 14, 2022
Page updated: January 21, 2023
Page reviewed: January 21, 2023
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external