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Volume 29, Number 2—February 2023
Research

Novel Prion Strain as Cause of Chronic Wasting Disease in a Moose, Finland

Julianna L. Sun, Sehun Kim, Jenna Crowell, Bailey K. Webster, Emma K. Raisley, Diana C. Lowe, Jifeng Bian, Sirkka-Liisa Korpenfelt, Sylvie L. Benestad, and Glenn C. TellingComments to Author 
Author affiliations: Colorado State University, Fort Collins, Colorado, USA (J.L. Sun, S. Kim, J. Crowell, B.K. Webster, E.K. Raisley, D.C. Lowe, J. Bian, G.C. Telling); Finnish Food Authority, Helsinki, Finland (S.-L. Korpenfelt); Norwegian Veterinary Institute, Ås, Norway (S.L. Benestad)

Main Article

Figure 3

Global central nervous system distribution of cervid prion protein scrapie in Tg and Gt mice infected with Finland, Norway, and North America chronic wasting disease (CWD) cryostat coronal brain sections taken at the level of the hippocampus, midbrain, pons, and oblongata, transferred to slides and then to nitrocellulose. Sections were proteinase K–treated and immunoprobed with monoclonal antibody PRC5 after denaturation. A, B) Passage 1 and 2 of M-F1 in GtQ mice. C) TgQ mice infected with M-F1. D) GtQ mice infected with Norway M-NO1 CWD. E) GtQ mice infected with CWD isolate M-US1. Gt, gene-targeted; GtQ, CWD-susceptible gene-targeted mice in which the prion protein coding sequence was replaced with one encoding glutamine at codon 226; M-F1: Finland moose 1; M-NO1, Norway moose 1; M-US1, US moose 1; p1, primary transmissions; p2, secondary transmissions; Tg, transgenic; TgQ, transgenic mice expressing cervid PrP with glutamine at residue 226.

Figure 3. Global central nervous system distribution of cervid prion protein scrapie in Tg and Gt mice infected with Finland, Norway, and North America chronic wasting disease (CWD) cryostat coronal brain sections taken at the level of the hippocampus, midbrain, pons, and oblongata, transferred to slides and then to nitrocellulose. Sections were proteinase K–treated and immunoprobed with monoclonal antibody PRC5 after denaturation. A, B) Passage 1 and 2 of M-F1 in GtQ mice. C) TgQ mice infected with M-F1. D) GtQ mice infected with Norway M-NO1 CWD. E) GtQ mice infected with CWD isolate M-US1. Gt, gene-targeted; GtQ, CWD-susceptible gene-targeted mice in which the prion protein coding sequence was replaced with one encoding glutamine at codon 226; M-F1: Finland moose 1; M-NO1, Norway moose 1; M-US1, US moose 1; p1, primary transmissions; p2, secondary transmissions; Tg, transgenic; TgQ, transgenic mice expressing cervid PrP with glutamine at residue 226.

Main Article

Page created: December 14, 2022
Page updated: January 21, 2023
Page reviewed: January 21, 2023
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