Volume 30, Number 8—August 2024
Synopsis
Outbreak of Intermediate Species Leptospira venezuelensis Spread by Rodents to Cows and Humans in L. interrogans–Endemic Region, Venezuela
Table 3
MLST of Leptospira interrogans isolates in study of outbreak of intermediate species L. venezuelensis spread by rodents to cows and humans in L. interrogans–endemic region, Venezuela*
| Isolates† | MLST allele nos. |
ST‡ | ||||||
|---|---|---|---|---|---|---|---|---|
| glmU | pntA | sucA | tpiA | pfkB | mreA | caiB | ||
| Human | ||||||||
| CAB-H41 | 1 | 1 | 2 | 1 | 7 | 7 | 8 | NP* |
| CAY-U48 | 1 | 1 | 2 | 1 | 7 | 4 | 3 | 20 |
| CAB-U03 | 1 | 1 | 2 | 2 | 7 | 4 | 3 | NP |
| MAC-H04 | 1 | 1 | 2 | 2 | 7 | 4 | 5 | NP |
| URI-U06 | 1 | 1 | 3 | 2 | 7 | 4 | 3 | NP |
| URI-H01 | 1 | 1 | 3 | 2 | 7 | 4 | 3 | NP |
| CLM-H09 | 1 | 1 | 3 | 2 | 4 | 7 | 5 | NP |
| CLM-U30 | 1 | 3 | 2 | 2 | 4 | 4 | 19 | NP |
| MAC-H63 | 1 | 3 | 2 | 2 | 7 | 7 | 19 | NP |
| CAY-H65 | 1 | 3 | 3 | 1 | 4 | 5 | 5 | NP |
| SOB-U13 | 1 | 12 | 3 | 3 | 10 | 4 | 5 | NP |
| MAQ-U18 | 1 | 12 | 3 | 3 | 10 | 5 | 19 | NP |
| CLM-U22 | 1 | 12 | 2 | 3 | 10 | 6 | 19 | NP |
| CLM-U28 | 1 | 12 | 3 | 3 | 10 | 6 | 19 | 27 |
| CLM-H08 | 1 | 12 | 3 | 3 | 10 | 6 | 19 | 27 |
| GUA-H40 | 1 | 12 | 3 | 3 | 10 | 6 | 19 | 27 |
| CLM-U45 | 3 | 3 | 3 | 2 | 4 | 5 | 5 | NP |
| CLM-U47 | 3 | 3 | 3 | 3 | 4 | 5 | 5 | 37 |
| NAG-U02 | 6 | 1 | 3 | 2 | 4 | 7 | 3 | NP |
| CAY-U49 | 6 | 1 | 3 | 3 | 76 | 7 | 3 | NP |
| CLM-U46 | 6 | 2 | 3 | 3 | 7 | 7 | 19 | NP |
| CLM-U24 | 6 | 1 | 3 | 12 | 4 | 5 | 5 | NP |
| GUA-H52 | 6 | 3 | 2 | 2 | 4 | 4 | 3 | NP |
| GUA-H64 | 6 | 3 | 2 | 3 | 4 | 7 | 5 | NP |
| CAB-U11 | 6 | 3 | 3 | 2 | 4 | 5 | 5 | NP |
| GUA-H21 | 6 | 3 | 3 | 3 | 1 | 7 | 5 | NP |
| CAO-U23 | 6 | 3 | 3 | 3 | 4 | 5 | 19 | NP |
| MAQ-H53 | 6 | 8 | 2 | 2 | 9 | 7 | 5 | 50 |
| MAQ-H60 |
6 |
8 |
2 |
2 |
9 |
7 |
5 |
50 |
| Rat | ||||||||
| CLM-R09-A | 1 | 1 | 2 | 2 | 7 | 4 | 8 | NP |
| CLM-R11-A | 1 | 1 | 3 | 3 | 4 | 6 | 19 | NP |
| SOB-R13-B§ | 1 | 12 | 3 | 3 | 10 | 6 | 19 | 27 |
*Bold font indicates isolates that had identical profiles. MLST, multilocus sequence typing; NP, not present in database; ST, sequence type. †The first 3 letters for each isolate indicate the area of the patient’s residence or where the rodent was captured in the state of La Guaira, Venezuela: CAB, Caraballeda; CAO, Caruao; CAY, Carayaca; CLM, Catia La Mar; GUA, La Guaira; MAC, Macuto; MAQ, Maiquetia; NAG, Niguata; SOB, Soublette; or URI, Urimare. The fourth letter is H (isolated from human blood), U (isolated from human urine), or R (isolated from rat tissue). ‡STs found in the Leptospira PubMLST database (https://pubmlst.org/organisms/leptospira-spp) (14). §Rat sequence shared an MLST profile for some alleles with human isolates CLM-U28, CLM-H08, and GUA-H40.
1Current affiliation: Universidad de las Fuerzas Armadas ESPE, Santo Domingo, Ecuador.
2Current affiliation: Universidad de Buenos Aires, Buenos Aires, Argentina.
3Current affiliation: Institut Pasteur de Montevideo, Montevideo, Uruguay.
4Current affiliation: The University of North Carolina, Chapel Hill, North Carolina, USA.