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Volume 31, Number 12—December 2025

Research

Silent Propagation of Classical Scrapie Prions in Homozygous K222 Transgenic Mice

Natalia Fernández-Borges1, Alba Marín-Moreno1, Juan Carlos Espinosa, Sara Canoyra, Olivier Andréoletti, and Juan María TorresComments to Author 
Author affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA-CSIC), Madrid, Spain (N. Fernández-Borges, A. Marín-Moreno, J.C. Espinosa, S. Canoyra, J.M. Torres); Interactions Hôte Agent Pathogène–École Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti)

Main Article

Figure 1

Proteinase K–resistant PrP (PrPres) accumulation in brains of K222-Tg516 and Q222-Tg501 homozygous mice in study of propagation of classical scrapie prions. A) Comparison of the biochemical profile of brain PrPres from classical scrapie isolates in K222-Tg516 mice with that in Q222-Tg501 mice using Sha31 monoclonal antibody. Exposure time and dilution factor are specified. B) Comparison of the biochemical profile of brain PrPres of CP060146 and F10 isolates of classical scrapie, before (left) and after (right) adaptation to the K222-PrPC context, in Q222-Tg501 mice, using the Sha31 monoclonal antibody. Molecular weight markers are indicated on the right side of each band.

Figure 1. Proteinase K–resistant PrP (PrPres) accumulation in brains of K222-Tg516 and Q222-Tg501 homozygous mice in study of propagation of classical scrapie prions. A) Comparison of the biochemical profile of brain PrPres from classical scrapie isolates in K222-Tg516 mice with that in Q222-Tg501 mice using Sha31 monoclonal antibody. Exposure time and dilution factor are specified. B) Comparison of the biochemical profile of brain PrPres of CP060146 and F10 isolates of classical scrapie, before (left) and after (right) adaptation to the K222-PrPC context, in Q222-Tg501 mice, using the Sha31 monoclonal antibody. Molecular weight markers are indicated on the right side of each band.

Main Article

1These first authors contributed equally to this article.

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Page updated: January 01, 2026
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