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Volume 31, Number 12—December 2025

Research

Silent Propagation of Classical Scrapie Prions in Homozygous K222 Transgenic Mice

Natalia Fernández-Borges1, Alba Marín-Moreno1, Juan Carlos Espinosa, Sara Canoyra, Olivier Andréoletti, and Juan María TorresComments to Author 
Author affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA-CSIC), Madrid, Spain (N. Fernández-Borges, A. Marín-Moreno, J.C. Espinosa, S. Canoyra, J.M. Torres); Interactions Hôte Agent Pathogène–École Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti)

Main Article

Figure 7

Kinetic studies of proteinase K–resistant prion protein (PrPres) detection in K222-Tg516 and Q222-Tg501 homozygous mice inoculated with classical scrapie in study of propagation of classical scrapie prions. Brain PrPres from mice euthanized at various time points postinoculation were analyzed by Western blotting and visualized using the Sha31 monoclonal antibody. A) Q222-Tg501 and K222-Tg516 mice inoculated with the CP060146 classical scrapie isolate adapted to the K222 cellular prion protein (PrPC) context (CP060146/K222-goat). B) Q222-Tg501 and K222-Tg516 mice inoculated with the F10 classical scrapie isolate adapted to the K222-PrPC context (F10/K222-Tg516). Molecular weight markers are indicated on the right side of each band.

Figure 7. Kinetic studies of proteinase K–resistant prion protein (PrPres) detection in K222-Tg516 and Q222-Tg501 homozygous mice inoculated with classical scrapie in study of propagation of classical scrapie prions. Brain PrPres from mice euthanized at various time points postinoculation were analyzed by Western blotting and visualized using the Sha31 monoclonal antibody. A) Q222-Tg501 and K222-Tg516 mice inoculated with the CP060146 classical scrapie isolate adapted to the K222 cellular prion protein (PrPC) context (CP060146/K222-goat). B) Q222-Tg501 and K222-Tg516 mice inoculated with the F10 classical scrapie isolate adapted to the K222-PrPC context (F10/K222-Tg516). Molecular weight markers are indicated on the right side of each band.

Main Article

1These first authors contributed equally to this article.

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