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Volume 31, Number 12—December 2025

Research

Silent Propagation of Classical Scrapie Prions in Homozygous K222 Transgenic Mice

Natalia Fernández-Borges1, Alba Marín-Moreno1, Juan Carlos Espinosa, Sara Canoyra, Olivier Andréoletti, and Juan María TorresComments to Author 
Author affiliation: Centro de Investigación en Sanidad Animal (CISA-INIA-CSIC), Madrid, Spain (N. Fernández-Borges, A. Marín-Moreno, J.C. Espinosa, S. Canoyra, J.M. Torres); Interactions Hôte Agent Pathogène–École Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti)

Main Article

Figure 6

Proteinase K digestion studies conducted as part of study of propagation of classical scrapie prions. K222-Tg516 and Q222-Tg501 homozygous mice were inoculated with classical scrapie. A) Proteinase K–resistant prion protein (PrPres) sensitivity in the brains of Q222-Tg501 and K222-Tg516 mice initially inoculated with F10 scrapie isolate and subsequently reinoculated into both the original model and its counterpart. B) PrPres sensitivity in the brains of Q222-Tg501 and K222-Tg516 mice initially inoculated with CP060146 scrapie isolate and subsequently reinoculated into both the original model and its counterpart. In both cases, proteinase K concentrations of 500, 50, 1 and 0.1 µg/mL were tested. Western blot visualizations were done using the Sha31 monoclonal antibody. Molecular weight markers are indicated on the right side of each band.

Figure 6. Proteinase K digestion studies conducted as part of study of propagation of classical scrapie prions. K222-Tg516 and Q222-Tg501 homozygous mice were inoculated with classical scrapie. A) Proteinase K–resistant prion protein (PrPres) sensitivity in the brains of Q222-Tg501 and K222-Tg516 mice initially inoculated with F10 scrapie isolate and subsequently reinoculated into both the original model and its counterpart. B) PrPres sensitivity in the brains of Q222-Tg501 and K222-Tg516 mice initially inoculated with CP060146 scrapie isolate and subsequently reinoculated into both the original model and its counterpart. In both cases, proteinase K concentrations of 500, 50, 1 and 0.1 µg/mL were tested. Western blot visualizations were done using the Sha31 monoclonal antibody. Molecular weight markers are indicated on the right side of each band.

Main Article

1These first authors contributed equally to this article.

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