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Volume 32, Supplement –Summer 2026

SUPPLEMENT ISSUE
Supplement

Evaluation of Detection Methods for Wastewater Surveillance of Antimicrobial-Resistant Bacteria from Healthcare Facilities

Ean Warren, James VanDerslice, L. Scott Benson, William J. Brazelton, Windy Tanner, Amanda K. Lyons, Florence Whitehill, Angela Coulliette-Salmond, Sydney Fusco, and Jennifer WeidhaasComments to Author 
Author affiliation: University of Utah, Salt Lake City, Utah, USA (E. Warren, J. VanDerslice, L.S. Benson, W.J. Brazelton, W. Tanner, S. Fusco, J. Weidhaas); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (F. Whitehill, A. Coulliette-Salmond); US Public Health Service, Rockville, Maryland, USA (A. Coulliette-Salmond)

Main Article

Figure 1

Measured Ct (mean +SD), calculated log GC/mL, and extrapolated log CFU/mL for detection limit studies in study of detection methods for wastewater surveillance of antimicrobial-resistant bacteria from healthcare facilities. A) blaNDM; B) blaIMP; C) blaVIM. Each point represents 5 replicates. Horizontal line represents a Ct cutoff of 35 cycles. All data points were included in the linear regression for each plot; dashed lines represent 95% CIs of the regression. The log CFU/mL was extrapolated from the initial isolate concentration, which was then serially diluted by 6 log into each of 5 replicates. The log GC/mL was estimated from the Ct response from standard curves using either geneblock DNA or antimicrobial-resistant isolates as positive controls. Ct, cycle threshold; GC, gene copies

Figure 1. Measured Ct (mean +SD), calculated log GC/mL, and extrapolated log CFU/mL for detection limit studies in study of detection methods for wastewater surveillance of antimicrobial-resistant bacteria from healthcare facilities. A) blaNDM; B) blaIMP; C) blaVIM. Each point represents 5 replicates. Horizontal line represents a Ct cutoff of 35 cycles. All data points were included in the linear regression for each plot; dashed lines represent 95% CIs of the regression. The log CFU/mL was extrapolated from the initial isolate concentration, which was then serially diluted by 6 log into each of 5 replicates. The log GC/mL was estimated from the Ct response from standard curves using either geneblock DNA or antimicrobial-resistant isolates as positive controls. Ct, cycle threshold; GC, gene copies

Main Article

Page created: May 26, 2026
Page updated: September 15, 2026
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