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Volume 32, Number 9—September 2026

Research

Clustering and Source Association of Clinical and Nonclinical Listeria monocytogenes Isolates, New York, USA, 2000–20211

Hilal Samut, Damaris V. Mendez-Vallellanes, Hannah Hoyt, Samantha E. Wirth, Lisa Mingle, Brian D. Sauders, Gregory A. Deiulio, Alyssa W. Dickey, Maria L. Ishida, William J. Wolfgang, Kimberlee A. Musser, Martin Wiedmann, and Renato H. OrsiComments to Author 
Author affiliation: Cornell University, Ithaca, New York, USA (H. Samut, M. Wiedmann, R.H. Orsi); New York State Department of Health, Wadsworth Center, Albany, New York, USA (D.V. Mendez-Vallellanes, H. Hoyt, S.E. Wirth, L. Mingle, W.J. Wolfgang, K.A. Musser); New York State Department of Agriculture and Markets, Albany (B.D. Sauders, G.A. Deiulio, A.W. Dickey, M.L. Ishida)

Main Article

Table 5

Characteristics of food-clinical Listeria monocytogenes isolate pairs used in the analysis of time between food isolate collection and subsequent detection of a genetically related clinical isolate by SNP cluster in study of clustering and source association of clinical and nonclinical L. monocytogenes isolates, New York, USA, 2000–2021*

CC SNP cluster Food category No. food–clinical isolate pairs† No. distinct food isolates No. distinct clinical isolates linked with food isolates by<50 SNPs inlA PMSC types in food and clinical isolates‡
CC1 PDS000003335§ Processed dairy 1 1 1 None
PDS000025547 Processed meat 3 1 3 None
PDS000082531 Processed dairy 1 1 1 None

PDS000091530
Raw dairy
1
1
1
None
CC5 PDS000024625 Processed dairy 1 1 1 None

PDS000024647
Salad
7
1
7
None
CC6 PDS000025014 Processed dairy 5 1 5 None

PDS000025078§
Processed meat
23
1
23
None
CC7
PDS000003277
Processed meat
3
1
3
None
CC8 PDS000025311 Raw dairy 2 1 2 None


Processed fish
1
1
1
None
CC88
PDS000003204
Processed fish
3
1
3
None
CC121
PDS000024645
Processed fish
2
2
1
Type 6
CC155 PDS000024385 Salad¶ 1 1 1 None

PDS000024989§
Raw meat¶
13
1
13
None
CC199 PDS000083553# Processed fish 2 2 1 Type 4


Processed meat
1
1
1
Type 4
CC204
PDS000003073
Salad¶
2
1
2
None
CC226
PDS000025453
Raw dairy
1
1
1
None
CC321 PDS000000366§ Processed fish 7 5 3 Type 3


Processed meat¶
96
9
12
Type 3
CC369
PDS000090530
Produce
1
1
1
None
CC388
PDS000043055
Raw dairy
1
1
1
None
CC389
PDS000024852
Raw dairy
1
1
1
None
CC554 PDS000003170 Processed fish 8 1 8 None

PDS000003173
Raw dairy
6
1
6
None
CC573 PDS000000308 Produce 12 3 5 None

*CC, clonal complex; PMSC, premature stop codon; SNP, single-nucleotide polymorphism. †Not all food–clinical isolate pairs met the <50 SNP threshold and, therefore, the number of food–clinical isolate pairs is not necessarily equal the number of food isolates times the number of clinical isolates in each SNP cluster. ‡When detected, inlA PMSC was present in all food and clinical isolates used in this analysis within the corresponding SNP cluster. When inlA PMSC was not detected, it was indicated as none. §Indicates SNP clusters that had formal outbreak investigations. In our analysis, PDS000003335 had 1 clinical isolate that was linked with 1 processed dairy isolate (by 2 SNPs); PDS000025078 had 9 clinical isolates that were linked with 1 processed meat isolate (3–9 SNPs); PDS000024989 had 2 clinical isolates that were linked with 1 raw meat isolate (0–2 SNPs), and PDS000000366 had 4r clinical isolates that were linked with 9 processed meat isolates (2–50 SNPs). Among those, 2 outbreak investigations (for the isolates in PDS000025078 and PDS000000366) had processed meat as the implicated food source, whereas the remaining investigations did not have a confirmed source. ¶These SNP clusters had other food categories matching to the clinical isolates by <50 SNPs. For clinical isolates associated with multiple food categories, only the clinical–food isolate pairs with the lowest pairwise SNP distance were retained. #For this SNP cluster, 2 food isolates from different food categories had the same minimum SNP distance to the same clinical isolate, and both food-clinical isolate pairs were retained.

Main Article

1Preliminary results from this study were presented at the 2025 International Association for Food Protection Conference; July 27–30, 2025; Cleveland, Ohio, USA.

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Page updated: August 17, 2026
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