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Volume 27, Number 5—May 2021
Research

Susceptibility to SARS-CoV-2 of Cell Lines and Substrates Commonly Used to Diagnose and Isolate Influenza and Other Viruses

Li Wang, Xiaoyu Fan, Gaston Bonenfant, Dan Cui, Jaber Hossain, Nannan Jiang, Gloria Larson, Michael Currier, Jimma Liddell, Malania Wilson, Azaibi Tamin, Jennifer Harcourt, Jessica Ciomperlik-Patton, Hong Pang, Naomi Dybdahl-Sissoko, Ray Campagnoli, Pei-Yong Shi, John Barnes, Natalie J. Thornburg, David E. Wentworth, and Bin ZhouComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (L. Wang, X. Fan, J. Hossain, M. Currier, M. Wilson, A. Tamin, J. Harcourt, J. Ciomperlik-Patton, H. Pang, N. Dybdahl-Sissoko, R. Campagnoli, J. Barnes, N.J. Thornburg, D.E. Wentworth, B. Zhou); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (G. Bonenfant, N. Jiang, G. Larson); Battelle Memorial Institute, Atlanta, Georgia, USA (D. Cui, J. Liddell); University of Texas Medical Branch, Galveston, Texas, USA (P.-Y. Shi)

Main Article

Figure 4

Poliovirus and enterovirus substrates not infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in study of susceptibility to SARS-CoV-2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. Total viral RNA levels determined by real-time reverse transcription PCR (standard curve generated by synthetic RNA) from RNA extracted from cell lines inoculated with USA-WA1 at MOI 0.1 in 6-well plates. Data points at 1 h represented by RNA from the inoculum; >2 h time points from RNA extracted from cell lysates. Data are mean of n = 3 +SD.

Figure 4. Poliovirus and enterovirus substrates not infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in study of susceptibility to SARS-CoV-2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. Total viral RNA levels determined by real-time reverse transcription PCR (standard curve generated by synthetic RNA) from RNA extracted from cell lines inoculated with USA-WA1 at MOI 0.1 in 6-well plates. Data points at 1 h represented by RNA from the inoculum; >2 h time points from RNA extracted from cell lysates. Data are mean of n = 3 +SD.

Main Article

Page created: February 24, 2021
Page updated: April 20, 2021
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