Susceptibility to SARS-CoV-2 of Cell Lines and Substrates Commonly Used to Diagnose and Isolate Influenza and Other Viruses
Li Wang, Xiaoyu Fan, Gaston Bonenfant, Dan Cui, Jaber Hossain, Nannan Jiang, Gloria Larson, Michael Currier, Jimma Liddell, Malania Wilson, Azaibi Tamin, Jennifer Harcourt, Jessica Ciomperlik-Patton, Hong Pang, Naomi Dybdahl-Sissoko, Ray Campagnoli, Pei-Yong Shi, John Barnes, Natalie J. Thornburg, David E. Wentworth, and Bin Zhou
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (L. Wang, X. Fan, J. Hossain, M. Currier, M. Wilson, A. Tamin, J. Harcourt, J. Ciomperlik-Patton, H. Pang, N. Dybdahl-Sissoko, R. Campagnoli, J. Barnes, N.J. Thornburg, D.E. Wentworth, B. Zhou); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (G. Bonenfant, N. Jiang, G. Larson); Battelle Memorial Institute, Atlanta, Georgia, USA (D. Cui, J. Liddell); University of Texas Medical Branch, Galveston, Texas, USA (P.-Y. Shi)
Figure 3. Influenza virus substrates not infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in study of susceptibility to SARS-CoV-2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. A) Vero E6, MDCK-NBL-2, MDCK-Atlanta, MDCK-London, MDCK-SIAT1, MDCK-hCK, and chicken embryo fibroblast cells inoculated with USA-WA1 at 5 × 104 TCID50/well in 12-well plates (MOI 0.1 to ≈0.3, depending on cell line). B) USA-WA1 total viral RNA levels in allantoic fluid from infected eggs quantified by real-time reverse transcription PCR using a standard curve generated by synthetic RNA. Four eggs with undetectable RNA not plotted for E3. Data are mean of n = 3 +SD (cells) or n = 24 +SD (eggs). TCID50, median tissue culture infectious dose.
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