Susceptibility to SARS-CoV-2 of Cell Lines and Substrates Commonly Used to Diagnose and Isolate Influenza and Other Viruses
Li Wang, Xiaoyu Fan, Gaston Bonenfant, Dan Cui, Jaber Hossain, Nannan Jiang, Gloria Larson, Michael Currier, Jimma Liddell, Malania Wilson, Azaibi Tamin, Jennifer Harcourt, Jessica Ciomperlik-Patton, Hong Pang, Naomi Dybdahl-Sissoko, Ray Campagnoli, Pei-Yong Shi, John Barnes, Natalie J. Thornburg, David E. Wentworth, and Bin Zhou
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (L. Wang, X. Fan, J. Hossain, M. Currier, M. Wilson, A. Tamin, J. Harcourt, J. Ciomperlik-Patton, H. Pang, N. Dybdahl-Sissoko, R. Campagnoli, J. Barnes, N.J. Thornburg, D.E. Wentworth, B. Zhou); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (G. Bonenfant, N. Jiang, G. Larson); Battelle Memorial Institute, Atlanta, Georgia, USA (D. Cui, J. Liddell); University of Texas Medical Branch, Galveston, Texas, USA (P.-Y. Shi)
Figure 1. Select commercially sourced cell lines infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in study of susceptibility to SARS-CoV-2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. A) Vero; B) Vero 76; C) BGMK; D) CV-1; E) LLC-MK2; F) RhMK; G) A549; H) HEL; I) HeLa; J) Hela 229; K) Hep-2; L) MRC-5; M) MRHF; N) NCI-H292; O) RD; P) WI-38; Q) McCoy; R) MNA; S) MDCK; T) CRFK; U) Mv1Lu; V) H&V-Mix; W) R-Mix; X) R-Mix Too; Y) Super E-Mix. Cell lines were inoculated with the SARS-CoV-2 reporter virus encoding mNeonGreen (icSARS-CoV-2-mNG) and infected cells (green fluorescence). Microscopy images (original magnification ×10) captured 1 day postinfection, but similar results were observed through 5 days postinfection; all mNeonGreen-negative cell lines remained negative.
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