Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 27, Number 5—May 2021
Research

Susceptibility to SARS-CoV-2 of Cell Lines and Substrates Commonly Used to Diagnose and Isolate Influenza and Other Viruses

Li Wang, Xiaoyu Fan, Gaston Bonenfant, Dan Cui, Jaber Hossain, Nannan Jiang, Gloria Larson, Michael Currier, Jimma Liddell, Malania Wilson, Azaibi Tamin, Jennifer Harcourt, Jessica Ciomperlik-Patton, Hong Pang, Naomi Dybdahl-Sissoko, Ray Campagnoli, Pei-Yong Shi, John Barnes, Natalie J. Thornburg, David E. Wentworth, and Bin ZhouComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (L. Wang, X. Fan, J. Hossain, M. Currier, M. Wilson, A. Tamin, J. Harcourt, J. Ciomperlik-Patton, H. Pang, N. Dybdahl-Sissoko, R. Campagnoli, J. Barnes, N.J. Thornburg, D.E. Wentworth, B. Zhou); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (G. Bonenfant, N. Jiang, G. Larson); Battelle Memorial Institute, Atlanta, Georgia, USA (D. Cui, J. Liddell); University of Texas Medical Branch, Galveston, Texas, USA (P.-Y. Shi)

Main Article

Figure 1

Select commercially sourced cell lines infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in study of susceptibility to SARS-CoV-2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. A) Vero; B) Vero 76; C) BGMK; D) CV-1; E) LLC-MK2; F) RhMK; G) A549; H) HEL; I) HeLa; J) Hela 229; K) Hep-2; L) MRC-5; M) MRHF; N) NCI-H292; O) RD; P) WI-38; Q) McCoy; R) MNA; S) MDCK; T) CRFK; U) Mv1Lu; V) H&V-Mix; W) R-Mix; X) R-Mix Too; Y) Super E-Mix. Cell lines were inoculated with the SARS-CoV-2 reporter virus encoding mNeonGreen (icSARS-CoV-2-mNG) and infected cells (green fluorescence). Microscopy images (original magnification ×10) captured 1 day postinfection, but similar results were observed through 5 days postinfection; all mNeonGreen-negative cell lines remained negative.

Figure 1. Select commercially sourced cell lines infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in study of susceptibility to SARS-CoV-2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. A) Vero; B) Vero 76; C) BGMK; D) CV-1; E) LLC-MK2; F) RhMK; G) A549; H) HEL; I) HeLa; J) Hela 229; K) Hep-2; L) MRC-5; M) MRHF; N) NCI-H292; O) RD; P) WI-38; Q) McCoy; R) MNA; S) MDCK; T) CRFK; U) Mv1Lu; V) H&V-Mix; W) R-Mix; X) R-Mix Too; Y) Super E-Mix. Cell lines were inoculated with the SARS-CoV-2 reporter virus encoding mNeonGreen (icSARS-CoV-2-mNG) and infected cells (green fluorescence). Microscopy images (original magnification ×10) captured 1 day postinfection, but similar results were observed through 5 days postinfection; all mNeonGreen-negative cell lines remained negative.

Main Article

Page created: February 24, 2021
Page updated: April 20, 2021
Page reviewed: April 20, 2021
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external