Volume 27, Number 5—May 2021
Research
Susceptibility to SARS-CoV-2 of Cell Lines and Substrates Commonly Used to Diagnose and Isolate Influenza and Other Viruses
Table 2
Primers and probes used for the quantification of ACE2 mRNA in various cell lines in study of susceptibility to SARS-CoV-2 of cell lines and substrates used to diagnose and isolate influenza and other viruses
Assay identification | Applicable cell lines | Primers/probes* | Sequence, 5′ → 3′ |
---|---|---|---|
ACE2.FAM.10 |
Vero E6, A549, CRFK, CV-1 |
Forward | CCCAGAATCCTTGAGTCAT |
Probe | TACTGATGCAATGGTGAACC | ||
Reverse |
TTGGACAGAAACCAAACATAG |
||
ACE2.FAM.11 |
Vero E6, CRFK |
Forward | GGGTCACAGTATGTTTCATC |
Probe | TATCTCTCGCTTCATCTCCC | ||
Reverse |
GGAGGTGGATGGTCTTTA |
||
ACE2.FAM.12 |
Vero E6, MDCK-NBL-2, MDCK-SIAT1 |
Forward | TGGTCTTTGGGAATTTCA |
Probe | TAAAGACCATCCACCTCCAC | ||
Reverse |
GAAATCATGTCACTTTCTGC |
||
ACE2.FAM.13 |
Vero E6, MDCK-NBL-2, MDCK-SIAT1 |
Forward | AACATGGAACAGAGATGC |
Probe | CCAAAGACCAGTGGATGAAA | ||
Reverse |
GGAGGTGGATGGTCTTTA |
||
ACE2.FAM.14 |
Vero E6, Mv1Lu |
Forward | CTTCATAGTCTCCTCTCCAATAA |
Probe | CTCTTCATATAATGGCCTCAGC | ||
Reverse |
CTACAATGAGAGGCTCTGG |
||
ACE2.FAM.15 |
Vero E6, Mv1Lu |
Forward | CTCTTCATATAATGGCCTCAG |
Probe | AGACTACAATGAGAGGCTCT | ||
Reverse |
ATGAGCACCATCTACAGT |
||
ACE2.FAM.16 | Vero E6, A549, CV-1 | Forward | GGGTCACAGTATGTTTCATC |
Probe | TATCTCTCGCTTCATCTCCC | ||
Reverse | GGAGGTGGATGGTCTTTA |
*Probes labeled at the 5′-end with the reporter molecule 6-carboxyfluorescein (FAM), internally with the quencher ZEN, and at the 3′-end with Iowa Black FQ (Integrated DNA Technologies, https://www.idtdna.com).