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Volume 27, Number 5—May 2021
Research

Susceptibility to SARS-CoV-2 of Cell Lines and Substrates Commonly Used to Diagnose and Isolate Influenza and Other Viruses

Li Wang, Xiaoyu Fan, Gaston Bonenfant, Dan Cui, Jaber Hossain, Nannan Jiang, Gloria Larson, Michael Currier, Jimma Liddell, Malania Wilson, Azaibi Tamin, Jennifer Harcourt, Jessica Ciomperlik-Patton, Hong Pang, Naomi Dybdahl-Sissoko, Ray Campagnoli, Pei-Yong Shi, John Barnes, Natalie J. Thornburg, David E. Wentworth, and Bin ZhouComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (L. Wang, X. Fan, J. Hossain, M. Currier, M. Wilson, A. Tamin, J. Harcourt, J. Ciomperlik-Patton, H. Pang, N. Dybdahl-Sissoko, R. Campagnoli, J. Barnes, N.J. Thornburg, D.E. Wentworth, B. Zhou); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (G. Bonenfant, N. Jiang, G. Larson); Battelle Memorial Institute, Atlanta, Georgia, USA (D. Cui, J. Liddell); University of Texas Medical Branch, Galveston, Texas, USA (P.-Y. Shi)

Main Article

Table 2

Primers and probes used for the quantification of ACE2 mRNA in various cell lines in study of susceptibility to SARS-CoV-2 of cell lines and substrates used to diagnose and isolate influenza and other viruses

Assay identification Applicable cell lines Primers/probes* Sequence, 5′ → 3′
ACE2.FAM.10
 Vero E6, A549, CRFK, CV-1
 Forward CCCAGAATCCTTGAGTCAT
Probe TACTGATGCAATGGTGAACC
Reverse
 TTGGACAGAAACCAAACATAG
ACE2.FAM.11
 Vero E6, CRFK
 Forward GGGTCACAGTATGTTTCATC
Probe TATCTCTCGCTTCATCTCCC
Reverse
 GGAGGTGGATGGTCTTTA
ACE2.FAM.12
 Vero E6, MDCK-NBL-2, MDCK-SIAT1
 Forward TGGTCTTTGGGAATTTCA
Probe TAAAGACCATCCACCTCCAC
Reverse
 GAAATCATGTCACTTTCTGC
ACE2.FAM.13
 Vero E6, MDCK-NBL-2, MDCK-SIAT1
 Forward AACATGGAACAGAGATGC
Probe CCAAAGACCAGTGGATGAAA
Reverse
 GGAGGTGGATGGTCTTTA
ACE2.FAM.14
 Vero E6, Mv1Lu
 Forward CTTCATAGTCTCCTCTCCAATAA
Probe CTCTTCATATAATGGCCTCAGC
Reverse
 CTACAATGAGAGGCTCTGG
ACE2.FAM.15
 Vero E6, Mv1Lu
 Forward CTCTTCATATAATGGCCTCAG
Probe AGACTACAATGAGAGGCTCT
Reverse
 ATGAGCACCATCTACAGT
ACE2.FAM.16 Vero E6, A549, CV-1 Forward GGGTCACAGTATGTTTCATC
Probe TATCTCTCGCTTCATCTCCC
Reverse GGAGGTGGATGGTCTTTA

*Probes labeled at the 5′-end with the reporter molecule 6-carboxyfluorescein (FAM), internally with the quencher ZEN, and at the 3′-end with Iowa Black FQ (Integrated DNA Technologies, https://www.idtdna.com).

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Page created: February 24, 2021
Page updated: April 20, 2021
Page reviewed: April 20, 2021
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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