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Volume 27, Number 5—May 2021
Research

Susceptibility to SARS-CoV-2 of Cell Lines and Substrates Commonly Used to Diagnose and Isolate Influenza and Other Viruses

Li Wang, Xiaoyu Fan, Gaston Bonenfant, Dan Cui, Jaber Hossain, Nannan Jiang, Gloria Larson, Michael Currier, Jimma Liddell, Malania Wilson, Azaibi Tamin, Jennifer Harcourt, Jessica Ciomperlik-Patton, Hong Pang, Naomi Dybdahl-Sissoko, Ray Campagnoli, Pei-Yong Shi, John Barnes, Natalie J. Thornburg, David E. Wentworth, and Bin ZhouComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (L. Wang, X. Fan, J. Hossain, M. Currier, M. Wilson, A. Tamin, J. Harcourt, J. Ciomperlik-Patton, H. Pang, N. Dybdahl-Sissoko, R. Campagnoli, J. Barnes, N.J. Thornburg, D.E. Wentworth, B. Zhou); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (G. Bonenfant, N. Jiang, G. Larson); Battelle Memorial Institute, Atlanta, Georgia, USA (D. Cui, J. Liddell); University of Texas Medical Branch, Galveston, Texas, USA (P.-Y. Shi)

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Figure 6

ACE2 differentially expressed across cell lines in study of susceptibility to severe acute respiratory syndrome coronavirus 2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. A) Mock transfected 293T cells or 293T cells transfected with plasmids expressing human, dog, cat, or mink ACE2 immunoblotted for ACE2 protein expression. B) Whole-cell lysate from uninoculated Vero E6, CV-1, A549, Mv1Lu, CRFK, MDCK-NBL-2, and MDCK-SIAT1 cell lines immunoblotted for endogenous ACE2 expression. Recombinant human ACE2 used as a positive control for detecting human ACE2. C) Relative ACE2 expression determined by real-time quantitative PCR. Data are mean of n = 6 +SD. Boxes are 1 SD away from the mean, and whiskers indicate the minimum and maximum. ACE, angiotensin-converting enzyme 2.

Figure 6. ACE2 differentially expressed across cell lines in study of susceptibility to severe acute respiratory syndrome coronavirus 2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. A) Mock transfected 293T cells or 293T cells transfected with plasmids expressing human, dog, cat, or mink ACE2 immunoblotted for ACE2 protein expression. B) Whole-cell lysate from uninoculated Vero E6, CV-1, A549, Mv1Lu, CRFK, MDCK-NBL-2, and MDCK-SIAT1 cell lines immunoblotted for endogenous ACE2 expression. Recombinant human ACE2 used as a positive control for detecting human ACE2. C) Relative ACE2 expression determined by real-time quantitative PCR. Data are mean of n = 6 +SD. Boxes are 1 SD away from the mean, and whiskers indicate the minimum and maximum. ACE, angiotensin-converting enzyme 2.

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Page created: February 24, 2021
Page updated: April 20, 2021
Page reviewed: April 20, 2021
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