Volume 27, Number 5—May 2021
Research
Susceptibility to SARS-CoV-2 of Cell Lines and Substrates Commonly Used to Diagnose and Isolate Influenza and Other Viruses
Figure 8

Figure 8. MDCK-vector, MDCK-hACE2, and MDCK-cACE2 cells inoculated with USA-WA1 at 5 × 105 TCID50/well in 12-well plates in study of susceptibility to severe acute respiratory syndrome coronavirus 2 of cell lines and substrates used to diagnose and isolate influenza and other viruses. Supernatants collected at the indicated times. A) Viral titers determined by TCID50 assay; B) total viral RNA determined using real-time reverse transcription PCR (standard curve generated by synthetic RNA). Data for both panels are mean of n = 3 +SD. ACE, angiotensin-converting enzyme 2; cACE2, canine ACE2; hACE2, human ACE2; TCID50, median tissue culture infectious dose.
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