Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link

Volume 31, Number 12—December 2025

Research

Guinea Pig Model for Lassa Virus Infection of Reproductive Tract and Considerations for Sexual and Vertical Transmission

Josilene N. Seixas, Joy M. Gary1, Stephen R. Welch, Sarah C. Genzer, JoAnn D. Coleman-McCray, Jessica R. Harmon, Christina F. Spiropoulou, Luciana Silva-Flannery, Georgia Ficarra, Elizabeth Lee, Julu Bhatnagar, Jessica R. Spengler, and Jana M. RitterComments to Author 
Author affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article

Figure 6

Histopathologic findings and detection of Lassa virus (LASV) antigens and RNA in mammary tissues by immunohistochemistry (IHC) and in situ hybridization (ISH) in strain 13/N guinea pigs lethally infected with LASV strain Josiah in study of guinea pig model for LASV infection of reproductive tract and considerations for sexual and vertical transmission. A) Mammary skin (23 dpi), hematoxylin and eosin stain. Mild interstitial lymphoplasmacytic infiltrates and edema (asterisk) in the superficial dermis. B) Mammary gland (25 dpi), hematoxylin and eosin stain. Mild interstitial lymphoplasmacytic infiltrates (arrows) in the periglandular stroma. C) Mammary skin (23 dpi), IHC. Staining in keratinocytes of the epidermis. D) Mammary gland (23 dpi), ISH. Staining around vessels and within the stroma between glands (arrow) and within cells in a mammary duct lumen (asterisk). Original magnifications ×10 (panels A–C), ×20 (panel D).

Figure 6. Histopathologic findings and detection of Lassa virus (LASV) antigens and RNA in mammary tissues by immunohistochemistry (IHC) and in situ hybridization (ISH) in strain 13/N guinea pigs lethally infected with LASV strain Josiah in study of guinea pig model for LASV infection of reproductive tract and considerations for sexual and vertical transmission. A) Mammary skin (23 dpi), hematoxylin and eosin stain. Mild interstitial lymphoplasmacytic infiltrates and edema (asterisk) in the superficial dermis. B) Mammary gland (25 dpi), hematoxylin and eosin stain. Mild interstitial lymphoplasmacytic infiltrates (arrows) in the periglandular stroma. C) Mammary skin (23 dpi), IHC. Staining in keratinocytes of the epidermis. D) Mammary gland (23 dpi), ISH. Staining around vessels and within the stroma between glands (arrow) and within cells in a mammary duct lumen (asterisk). Original magnifications ×10 (panels A–C), ×20 (panel D).

Main Article

1Current affiliation: StageBio, Frederick, Maryland, USA.

Page created: November 25, 2025
Page updated: January 01, 2026
Page reviewed: January 01, 2026
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external