Figure 3. Observations of biofilm formation in a study of Burkholderia pseudomallei virulence in mouse models. Burkholderia pseudomallei isolates were inoculated into Luria broth with 4% glycerol and incubated statically at 37°C for 24 hours. We removed planktonic growth and stained the remaining biofilm by using crystal violet staining then quantified by using 600 nm optical density. We compared the test panel (represented by black bars) with a hyper-biofilm producing strain (strain no. ATS2021, represented by blue bar) and a common strain used in the laboratory with low biofilm formation (strain no. K96243, represented by red bar). Data displayed are the averaged results of >3 biologic replicates with error bars representing the standard error of the mean. Red p values indicate statistical significance relative to strain K96243. Blue p values indicate significance relative to strain ATS202. OD₆₀₀, optical density at 600 nm.