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Virulence of Burkholderia pseudomallei Strains from Western Hemisphere and Africa in Mice
Christopher P. Klimko, J. Matthew Meinig, Kevin D. Mlynek, Nathaniel O. Rill, Sergei S. Biryukov, Jennifer L. Dankmeyer, Annette M. Gray, Jennifer Chua, Stephanie A. Halasahoris, Michael L. Davies, Brian A. Smith, Carlos I. Rodriguez-Negron, Christopher T. Braun, Elsie E. Martinez, Jade L. Spencer, David N. Dyer, Ondraya M. Frick, Marjorie E. Torres, Mindy G. Elrod, Jay E. Gee, Christopher A. Gulvik, Zachary P. Weiner, Ju Qiu, Joel A. Bozue, David DeShazer, and Christopher K. Cote
Author affiliation: US Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA (C.P. Klimko, J.M. Meinig, K.D. Mlynek, N.O. Rill, S.S. Biryukov, J.L. Dankmeyer, A.M. Gray, J. Chua, S.A. Halasahoris, M.L. Davies, B.A. Smith, C.I. Rodriguez-Negron, C.T. Braun, E.E. Martinez, J.L. Spencer, D.N. Dyer, O.M. Frick, M.E. Torres, J. Qiu, J.A Bozue, D. DeShazer, C.K. Cote); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M.G. Elrod, J.E. Gee, C.A. Gulvik, Z.P. Weiner)
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Figure 6
Figure 6. Pangenome analysis of Burkholderia pseudomallei strains from the Western Hemisphere and Africa in mouse models. We used panaroo version 1.5.2 (https://github.com/gtonkinhill/panaroo) for analysis of all 10 isolate genomes. The core genome size is 5,176 genes, and scoary version 1.6.16 (https://github.com/AdmiralenOla/Scoary) revealed 2 hypothetical protein encoding genes unique to the 3 virulent strains from Africa (GHD1A, GHC5E, BpNY2023b): an 84-residue encoding gene and a 262-residue encoding gene. The range, spread, median, and mean came from 100 permutations simulating genome addition and was plotted in R version 4.5.1 (The R Project for Statistical Computing, https://www.r-project.org) with refinements in inkscape v1.0.1 (https://inkscape.org). Purple represents pangenome; green represents core genome. IQR, interquartile range.
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