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Volume 32, Number 8—August 2026
Research
Virulence of Burkholderia pseudomallei Strains from Western Hemisphere and Africa in Mice
Christopher P. Klimko, J. Matthew Meinig, Kevin D. Mlynek, Nathaniel O. Rill, Sergei S. Biryukov, Jennifer L. Dankmeyer, Annette M. Gray, Jennifer Chua, Stephanie A. Halasahoris, Michael L. Davies, Brian A. Smith, Carlos I. Rodriguez-Negron, Christopher T. Braun, Elsie E. Martinez, Jade L. Spencer, David N. Dyer, Ondraya M. Frick, Marjorie E. Torres, Mindy G. Elrod, Jay E. Gee, Christopher A. Gulvik, Zachary P. Weiner, Ju Qiu, Joel A. Bozue, David DeShazer, and Christopher K. Cote
Table 2
LD50 estimations in BALB/c and C57BL/6 mice calculated on day 21 and day 60 post exposure to aerosolized Burkholderia pseudomallei strains from the Western Hemisphere and Africa*
| Strain | BALB/c mice 21-d LD50 | C57BL/6 mice 21-d LD50 | BALB/c mice 60-d LD50 | C57BL/6 mice 60-d LD50 |
|---|---|---|---|---|
| GHD1A | 3.5 (0.2–10.5) | 359.5 (65.9–1,487.9) | 2.6 (2.4–16.6)† | 113.4 (16.1–414.1) |
| BpNY2023b | 2.1 (1.7–12.0)† | 166.9 (52.4–515.1) | 2.1 (1.7–12.0)† | 79.4 (27.4–239.3) |
| AZ1999 | 215.4 (84.8–471.2) | 1,420.3 (822.1–7,224.2)† | 12.8 (4.2–35.6) | 438.7 (150.4–1,418.8) |
| VEN1976 | 67.3 (26.2–217.5) | 470.8 (155.8–1,485.2) | 12.1 (11.1–96.8)† | 108.2 (99.9–1,342.8)† |
| PB10007001 | 4.2 (2.1–8.9) | 396.1 (178.1–1,445.4) | 1.8 (1.5–8.4)† | 169.6 (44.2–383.7) |
| IL2014 | 490.6 (458.1–4,223.7)† | 448.6 (419.0–3,863.0)† | 38.6 (8.5–155.1) | 242.5 (111.6–581.6) |
| PR2013a | 73.9 (26.9–190.8) | 757.1 (486.6–9,123.9)† | 6.7 (1.2–7.6)† | 345.1 (132.7–1,172.8) |
| GHC5E | 3.0 (0.1–11.1) | 223.8 (97.1–526.2) | 1.4 (0.0–3.1) | 99.3 (42.3–228.8) |
| MS2020a | 14.7 (0.4–3,490.4) | 889.0 (235.6–13,773.1) | 4.5 (3.1–9.1) | 400.9 (125.5–2,708.2) |
| TX2004 | 6,869.3 (1,210.1–NC)† | 2,124.6 (1,004.3–8,201.9) | 88.0 (10.5–298.3)† | 580.6 (134.6–4,314.3) |
*Values are LD50 (95% CI). LD50, 50% lethal dose; NC, not calculated. †Because of the lack of response gradient across the tested doses, the model failed to provide confidence intervals for the LD50 estimate. However, we calculated LD50 confidence intervals on the basis of observed response rates,
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