Outbreaks of Neuroinvasive Astrovirus Associated with Encephalomyelitis, Weakness, and Paralysis among Weaned Pigs, Hungary
Ákos Boros, Mihály Albert, Péter Pankovics, Hunor Bíró, Patricia A. Pesavento, Tung Gia Phan, Eric Delwart, and Gábor Reuter
Author affiliations: ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary (A. Boros, P. Pankovics, G. Reuter); University of Pécs, Pécs (A. Boros, P. Pankovics, G. Reuter); Ceva Phylaxia Ltd., Budapest, Hungary (M. Albert); SHP Ltd., Kaposvár, Hungary (H. Bíró); University of California, Davis, California, USA (P.A. Pesavento); Blood Systems Research Institute, San Francisco, California, USA (T.G. Phan, E. Delwart); University of California, San Francisco (E. Delwart)
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Figure 3
Figure 3. Logarithmic graph of the viral copy numbers of porcine astrovirus type 3 (PoAstV-3) in different organs determined by SYBR Green–based quantitative reverse transcription PCR (RT-qPCR) of samples from 5 symptomatic newly weaned pigs (GD-1–5; see Table 1) from a farm in Hungary. All the samples, which were positive for PoAstV-3 only by nested RT-PCR, were found negative by quantitative RT-PCR. BS, brain stem; CNS, central nervous system; NM, nasal mucosa; SC-C/T/L, cervical, thoracic, or lumbar spinal cord; SG, salivary gland.
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