Outbreaks of Neuroinvasive Astrovirus Associated with Encephalomyelitis, Weakness, and Paralysis among Weaned Pigs, Hungary
Ákos Boros, Mihály Albert, Péter Pankovics, Hunor Bíró, Patricia A. Pesavento, Tung Gia Phan, Eric Delwart, and Gábor Reuter
Author affiliations: ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary (A. Boros, P. Pankovics, G. Reuter); University of Pécs, Pécs (A. Boros, P. Pankovics, G. Reuter); Ceva Phylaxia Ltd., Budapest, Hungary (M. Albert); SHP Ltd., Kaposvár, Hungary (H. Bíró); University of California, Davis, California, USA (P.A. Pesavento); Blood Systems Research Institute, San Francisco, California, USA (T.G. Phan, E. Delwart); University of California, San Francisco (E. Delwart)
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Figure 1
Figure 1. Genome map of the neuroinvasive PoAstV-3 strain NI-Brain/9-2016a/HUN (GenBank accession no. KY073229) from a symptomatic newly weaned pig from a farm in Hungary together with the location of RT-PCR products used for different astrovirus screening reactions and quantitative RT-PCR analyses. The black arrow indicates the possible localization of a ribosomal frame-shift during the synthesis of ORF1ab peptide. The first and last nucleotide positions of the ORFs are marked with numbers at the top and bottom of each box. ORF, open reading frame; PanAstV, panastrovirus; PoAstV-3, porcine astrovirus type 3; RT-PCR, reverse transcription PCR; UTR, untranslated region.
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