Volume 23, Number 12—December 2017
Research
Outbreaks of Neuroinvasive Astrovirus Associated with Encephalomyelitis, Weakness, and Paralysis among Weaned Pigs, Hungary
Table 2
Results of PoAstV-3 detection, histology, and ISH analyses using formalin-fixed, paraffin-embedded blocks of samples from 3 symptomatic newly weaned pigs from a farm in Hungary and samples from 2 other farms with symptomatic pigs*
| Farm ID | Collection year | Animal ID | FFPE block ID | Nested RT-PCR† |
Tissue samples | ISH‡ | |
|---|---|---|---|---|---|---|---|
| RdRp | Capsid | ||||||
| GD | 2016 | GD-1 | GD-1A | – (+) | – (+) | Spinal cord | + |
| Brainstem | + | ||||||
| Cerebellum | + | ||||||
| Medulla oblongata | - | ||||||
| GD-2 | GD-2A | – (–) | – (–) | Lymph node | – | ||
| Tonsil | – | ||||||
| Myocardium | – | ||||||
| Spleen | – | ||||||
| Thymus | – | ||||||
| 2015 | GD-11§ | GD-11A | – (+) | – (+) | Brainstem | + | |
| Cerebellum |
+ |
||||||
| Tázlár | 2011 | TAZ-1 | TAZ-1A | – (+) | – (+) | Hippocampus | – |
| Brainstem | + | ||||||
| TAZ-1B |
– (+) |
– (+) |
Spinal cord |
+ |
|||
| Balmazújváros | 2014 | BAM-1 | BAM-1A | – (–) | – (–) | Spinal cord | – |
| BAM-1B | – (+) | – (+) | Brainstem | + | |||
| Cerebellum | + | ||||||
*The screening RT-PCR primers are designed to either the RdRp or the capsid region of PoAstV-3. GD, index farm; ID, identification; ISH, in situ hybridization; PoAstV-3, porcine astrovirus type 3; RdRp: RNA-dependent RNA polymerase; RT-PCR, reverse transcription PCR; +, positive; –, negative.
†Symbols indicate the results of the first screening PCRs; symbols in parentheses indicate the results of the second (nested) RT-PCR. The results of nested RT-PCR refer to a mixture of tissues embedded into the total of 7 paraffin blocks.
‡Indicates results for neuroinvasive PoAstV-3.
§FFPE samples were the only specimens taken from this animal.