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Volume 23, Number 12—December 2017

Outbreaks of Neuroinvasive Astrovirus Associated with Encephalomyelitis, Weakness, and Paralysis among Weaned Pigs, Hungary

Ákos Boros, Mihály Albert, Péter Pankovics, Hunor Bíró, Patricia A. Pesavento, Tung Gia Phan, Eric Delwart, and Gábor ReuterComments to Author 
Author affiliations: ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary (A. Boros, P. Pankovics, G. Reuter); University of Pécs, Pécs (A. Boros, P. Pankovics, G. Reuter); Ceva Phylaxia Ltd., Budapest, Hungary (M. Albert); SHP Ltd., Kaposvár, Hungary (H. Bíró); University of California, Davis, California, USA (P.A. Pesavento); Blood Systems Research Institute, San Francisco, California, USA (T.G. Phan, E. Delwart); University of California, San Francisco (E. Delwart)

Main Article

Table 2

Results of PoAstV-3 detection, histology, and ISH analyses using formalin-fixed, paraffin-embedded blocks of samples from 3 symptomatic newly weaned pigs from a farm in Hungary and samples from 2 other farms with symptomatic pigs*

Farm ID Collection year Animal ID FFPE block ID Nested RT-PCR†
Tissue samples ISH‡
RdRp Capsid
GD 2016 GD-1 GD-1A – (+) – (+) Spinal cord +
Brainstem +
Cerebellum +
Medulla oblongata -
GD-2 GD-2A – (–) – (–) Lymph node
2015 GD-11§ GD-11A – (+) – (+) Brainstem +

Tázlár 2011 TAZ-1 TAZ-1A – (+) – (+) Hippocampus
Brainstem +

– (+)
– (+)
Spinal cord
Balmazújváros 2014 BAM-1 BAM-1A – (–) – (–) Spinal cord
BAM-1B – (+) – (+) Brainstem +
Cerebellum +

*The screening RT-PCR primers are designed to either the RdRp or the capsid region of PoAstV-3. GD, index farm; ID, identification; ISH, in situ hybridization; PoAstV-3, porcine astrovirus type 3; RdRp: RNA-dependent RNA polymerase; RT-PCR, reverse transcription PCR; +, positive; –, negative.
†Symbols indicate the results of the first screening PCRs; symbols in parentheses indicate the results of the second (nested) RT-PCR. The results of nested RT-PCR refer to a mixture of tissues embedded into the total of 7 paraffin blocks.
‡Indicates results for neuroinvasive PoAstV-3.
§FFPE samples were the only specimens taken from this animal.

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Page created: November 16, 2017
Page updated: November 16, 2017
Page reviewed: November 16, 2017
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