Volume 23, Number 12—December 2017
Research
Outbreaks of Neuroinvasive Astrovirus Associated with Encephalomyelitis, Weakness, and Paralysis among Weaned Pigs, Hungary
Ákos Boros, Mihály Albert, Péter Pankovics, Hunor Bíró, Patricia A. Pesavento, Tung Gia Phan, Eric Delwart, and Gábor Reuter
Table 1
Data on 5 symptomatic and 5 control newly weaned pigs from a farm in Hungary and results of PoAstV-3 screening by nested RT-PCR of samples collected during 2015–2017*
| Data |
Symptomatic animals† |
Asymptomatic control animals† |
|||||||||
| GD-1 |
GD-2 |
GD-3 |
GD-4 |
GD-5 |
GD-6 |
GD-7 |
GD-8 |
GD-9 |
GD-10 |
||
| Collection month | 2016 Mar | 2016 Mar | 2016 Jul | 2016 Jul | 2015 Nov | 2016 Jul | 2017 Jun | 2017 Jun | 2017 Jun | 2017 Jun | |
| Age, d | 25 | 25 | 25 | 25 | 35 | 35 | 25 | 25 | 25 | 35 | |
| Clinical signs (disease stage) |
PP
(1) |
PP
(1) |
PP
(3) |
PP
(3) |
PP (3) |
None |
None |
None |
None |
None |
|
| Brain stem |
+ |
+ |
+ |
+ |
+ |
– (–) |
– (–) |
– (–) |
– (–) |
– (–) |
|
| Spinal cord | |||||||||||
| Cervical | NA | NA | + | + | NA | NA | – (–) | – (–) | NA | NA | |
| Thoracic | NA | NA | + | + | NA | NA | – (–) | – (–) | NA | NA | |
| Lumbar |
+ |
NA |
+ |
+ |
+ |
– (–) |
– (–) |
– (–) |
NA |
NA |
|
| Nasal mucosa | – (+)‡ | +‡ | + | + | NA | – (–) | – (–)‡ | – (–)‡ | – (–)‡ | – (–)‡ | |
| Lung | NA | NA | + | + | NA | NA | NA | NA | NA | NA | |
| Tonsils | NA | – (–) | + | + | + | – (–) | NA | NA | NA | NA | |
| Salivary glands | NA | NA | – (+) | + | NA | NA | NA | NA | NA | NA | |
| Myocardium | NA | + | NA | NA | + | NA | NA | NA | NA | NA | |
| Feces | – (–) | NA | – (+) | – (+) | NA | NA | – (–) | – (–) | – (–) | – (–) | |
| Ileum |
NA |
NA |
– (–) |
– (+) |
– (–) |
– (–) |
NA |
NA |
NA |
NA |
|
| Lymph nodes | |||||||||||
| Mesenterial | NA | – (–) | – (–) | – (–) | NA | – (–) | NA | NA | NA | NA | |
| Submandibular |
NA |
NA |
– (+) |
+ |
NA |
NA |
NA |
NA |
NA |
NA |
|
| Urine | NA | NA | – (–) | – (–) | NA | NA | NA | NA | NA | NA | |
| Kidney | NA | NA | – (–) | – (–) | NA | NA | NA | NA | NA | NA | |
| Liver | NA | NA | – (+) | – (+) | NA | NA | NA | NA | NA | NA | |
| Spleen | NA | NA | – (–) | – (–) | NA | NA | NA | NA | NA | NA | |
| Serum | NA | NA | + | + | NA | NA | NA | NA | NA | NA | |
*We collected tissues from 5 affected pigs with encephalomyelitis and PP and 5 asymptomatic control animals from the index farm. The screening nested RT-PCR primers are designed to the RNA-dependent RNA polymerase region of PoAstV-3. NA, no available sample; PoAstV-3, porcine astrovirus type 3; PP, posterior paraplegia; RT-PCR, reverse transcription PCR; +, positive; −, negative.
†Symbols indicate results from first PCR reactions; symbols in parentheses indicate results from second (nested) RT-PCR reactions.
‡Nasal swab sample.
Page created: November 16, 2017
Page updated: November 16, 2017
Page reviewed: November 16, 2017
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
Content Credential