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Volume 31, Number 12—December 2025

Research

Oral Transmission of Classical Bovine Spongiform Encephalopathy in ARR/ARR Sheep

Alvina Huor1, Frederic Lantier1, Jean-Yves Douet, Severine Lugan, Naima Aron, Chloe Mesic, Herve Cassard, Tomás Barrio, Hugh Simmons, Isabelle Lantier, and Olivier AndreolettiComments to Author 
Author affiliation: University of Toulouse, ENVI, INRAE, IHAP, Toulouse, France (A. Huor, J.-Y. Douet, S. Lugan, N. Aron, C. Mesic, H. Cassard, T. Barrio, O. Andreoletti); National Research Institute for Agriculture, Food and Environment, Nouzilly, France (F. Lantier, I. Lantier); Animal and Plant Health Agency, Weybridge, UK (H. Simmons)

Main Article

Figure 4

Protein misfolding cyclic amplification seeding activity levels in leukocytes of orally challenged ARR/ARR and ARQ/ARQ sheep in study of oral transmission of classical bovine spongiform encephalopathy in ARR/ARR sheep. A) ARQ/ARQ sheep. B) ARR/ARR sheep. We euthanized sheep at 4 mpi, 10 mpi, or at the clinical stage of infection. Leukocytes from 5 mL of whole blood collected at each month from birth until euthanasia were used to seed quadruplicate protein misfolding cyclic amplification reactions with a 10% wt/vol serial dilution from 10−1 to 10−5. Each symbol represents a different animal. mpi, months postinoculation; neg, negative.

Figure 4. Protein misfolding cyclic amplification seeding activity levels in leukocytes of orally challenged ARR/ARR and ARQ/ARQ sheep in study of oral transmission of classical bovine spongiform encephalopathy in ARR/ARR sheep. A) ARQ/ARQ sheep. B) ARR/ARR sheep. We euthanized sheep at 4 mpi, 10 mpi, or at the clinical stage of infection. Leukocytes from 5 mL of whole blood collected at each month from birth until euthanasia were used to seed quadruplicate protein misfolding cyclic amplification reactions with a 10% wt/vol serial dilution from 10−1 to 10−5. Each symbol represents a different animal. mpi, months postinoculation; neg, negative.

Main Article

1These first authors contributed equally to this article.

Page created: November 18, 2025
Page updated: January 01, 2026
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