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Volume 31, Number 12—December 2025

Research

Oral Transmission of Classical Bovine Spongiform Encephalopathy in ARR/ARR Sheep

Alvina Huor1, Frederic Lantier1, Jean-Yves Douet, Severine Lugan, Naima Aron, Chloe Mesic, Herve Cassard, Tomás Barrio, Hugh Simmons, Isabelle Lantier, and Olivier AndreolettiComments to Author 
Author affiliation: University of Toulouse, ENVI, INRAE, IHAP, Toulouse, France (A. Huor, J.-Y. Douet, S. Lugan, N. Aron, C. Mesic, H. Cassard, T. Barrio, O. Andreoletti); National Research Institute for Agriculture, Food and Environment, Nouzilly, France (F. Lantier, I. Lantier); Animal and Plant Health Agency, Weybridge, UK (H. Simmons)

Main Article

Figure 6

Western blot detection of proteinase K–resistant core PrP in the brains of tgBov, tgMet, (tgVal), or tgMet/Val mice inoculated with ARR/ARR or ARQ/ARQ c-BSE isolates in a study of the oral transmission of c-BSE in ARR/ARR sheep. A) ARQ/ARQ sheep. B) ARR/ARR sheep. Original c-BSE isolates from ARQ/ARQ or ARR/ARR sheep and a scrapie isolate were included as controls. Proteinase K–resistant core PrP was detected by using the monoclonal Sha31 anti-PrP. c-BSE, classical bovine spongiform encephalopathy; pass, passage; PrP, prion protein; tgBov, transgenic mice bovine PrP–expressing mice; tgMet, transgenic mice expressing human Met129; tgMet/Val, transgenic mice expressing human Met/Val129; tgVal, transgenic mice expressing human Val129.

Figure 6. Western blot detection of proteinase K–resistant core PrP in the brains of tgBov, tgMet, (tgVal), or tgMet/Val mice inoculated with ARR/ARR or ARQ/ARQ c-BSE isolates in a study of the oral transmission of c-BSE in ARR/ARR sheep. A) ARQ/ARQ sheep. B) ARR/ARR sheep. Original c-BSE isolates from ARQ/ARQ or ARR/ARR sheep and a scrapie isolate were included as controls. Proteinase K–resistant core PrP was detected by using the monoclonal Sha31 anti-PrP. c-BSE, classical bovine spongiform encephalopathy; pass, passage; PrP, prion protein; tgBov, transgenic mice bovine PrP–expressing mice; tgMet, transgenic mice expressing human Met129; tgMet/Val, transgenic mice expressing human Met/Val129; tgVal, transgenic mice expressing human Val129.

Main Article

1These first authors contributed equally to this article.

Page created: November 18, 2025
Page updated: January 01, 2026
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